Nuclear Targeting of Gold Nanoparticles in Cancer Cells Induces DNA Damage, Causing Cytokinesis Arrest and Apoptosis

被引：525

作者：

Kang, Bin ^{[1
]}

Mackey, Megan A. ^{[1
]}

El-Sayed, Mostafa A. ^{[1
]}

机构：

[1] Georgia Inst Technol, Sch Chem & Biochem, Laser Dynam Lab, Atlanta, GA 30332 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2010年 / 132卷 / 05期

关键词：

PHOTOTHERMAL THERAPY; DIFFERENT SHAPES; SIZE; CARCINOMA; NANOCRYSTALS; DEPENDENCE; CHEMISTRY; MICE;

D O I：

10.1021/ja9102698

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

By properly conjugating gold nanoparticles with specific peptides, we were successful in selectively transporting them to the nuclei of cancer cells. Confocal microscopy images of DNA double-strand breaks showed that localization of gold nanoparticles at the nucleus of a cancer cell damages the DNA. Gold nanoparticle dark-field imaging of live cells in real time revealed that the nuclear targeting of gold nanoparticles specifically induces cytokinesis arrest in cancer cells, where binucleate cell formation occurs after mitosis takes place. Flow cytometry results indicated that the failure to complete cell division led to programmed cell death (apoptosis) in cancer cells. These results show that gold nanoparticles localized at the nuclei of cancer cells have important Implications in understanding the interaction between nanomaterials and living systems.

引用

页码：1517 / +

页数：5

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