Quartz crystal microbalance studies on bilirubin adsorption on self-assembled phospholipid bilayers

被引:12
作者
Yang, Zhengpeng [1 ]
Si, Shihui [1 ]
Zhang, Chunjing [1 ]
Song, Ge [1 ]
机构
[1] Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Peoples R China
基金
中国国家自然科学基金;
关键词
phospholipid bilayer; bilirubin; adsorption; quartz crystal microbalance;
D O I
10.1016/j.jcis.2006.09.041
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Bilirubin adsorption on self-assembled phospholipid bilayers was studied using quartz crystal microbalance, and factors influencing its adsorption such as pH, temperature, and solution ionic strength were discussed in detail. The results show the amount of adsorbed bilirubin on self-assembled phospholipid bilayers is small at higher temperature and large at higher pH and solution ionic strength, and the adsorption kinetic parameter estimated from the in situ frequency measurement is (1.8 +/- 10.27) x 10(6) M-1 (mean S.D.). With the present method, the desorption of adsorbed bilirubin caused by human serum albumin and the photoincluced decomposition of adsorbed bilirubin under light illumination were also examined. QCM measurement provides a useful method for monitoring the adsorption/desorption process of bilirubin on self-assembled phospholipid bilayers. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码:1 / 6
页数:6
相关论文
共 35 条
[1]   Development of new immunosensors for determination of contaminants in food [J].
Adányi, N ;
Váradi, M ;
Kim, N ;
Szendrö, I .
CURRENT APPLIED PHYSICS, 2006, 6 (02) :279-286
[2]   PLGA-micro sphere mediated clearance of bilirubin in temporarily hyperbilirubinemic rats: An alternate strategy for the treatment of experimental jaundice [J].
Ahmad, N ;
Arif, K ;
Faisal, SM ;
Neyaz, MK ;
Tayyab, S ;
Owais, M .
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2006, 1760 (02) :227-232
[3]   Study of a fluorometric-enzymatic method for bilirubin based on chemically modified bilirubin-oxidase and multivariate calibration [J].
Andreu, Y ;
Ostra, M ;
Ubide, C ;
Galbán, J ;
de Marcos, S ;
Castillo, JR .
TALANTA, 2002, 57 (02) :343-353
[4]   Adsorptive membranes for bilirubin removal [J].
Avramescu, ME ;
Sager, WFC ;
Borneman, Z ;
Wessling, M .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2004, 803 (02) :215-223
[5]   Characterization of polyethylenimine grafted and Cibacron Blue F3GA immobilized poly (hydroxyethylmethacrylate-co-glycydylmethacrylate) membranes and application to bilirubin removal from human serum [J].
Bayramoglu, G ;
Yalçin, E ;
Arica, MY .
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS, 2005, 264 (1-3) :195-202
[6]   Piezoelectric immunosensors for urine specimens of Chlamydia trachomatis employing quartz crystal microbalance microgravimetric analyses [J].
BenDov, I ;
Willner, I ;
Zisman, E .
ANALYTICAL CHEMISTRY, 1997, 69 (17) :3506-3512
[7]   pKa and aggregation of bilirubin:: Titrimetric and ultracentrifugation studies on water-soluble pegylated conjugates of bilirubin and fatty acids [J].
Boiadjiev, SE ;
Watters, K ;
Wolf, S ;
Lai, BN ;
Welch, WH ;
McDonagh, AF ;
Lightner, DA .
BIOCHEMISTRY, 2004, 43 (49) :15617-15632
[8]   Quartz crystal microbalance study of DNA immobilization and hybridization for nucleic acid sensor development [J].
Caruso, F ;
Rodda, E ;
Furlong, DF ;
Niikura, K ;
Okahata, Y .
ANALYTICAL CHEMISTRY, 1997, 69 (11) :2043-2049
[9]   BIOMEMBRANES AND NEW HEMOCOMPATIBLE MATERIALS [J].
CHAPMAN, D .
LANGMUIR, 1993, 9 (01) :39-45
[10]   The effect of pressure and temperature on the vibrational spectra of different hydrogen bonded systems [J].
Desseyn, HO ;
Clou, K ;
Keuleers, R ;
Miao, R ;
Van Doren, VE ;
Blaton, N .
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2001, 57 (02) :231-246