Organophosphorus and carbamate pesticide analysis using an inhibition tyrosinase organic phase enzyme sensor; comparison by butyrylcholinesterase plus choline oxidase opee and application to natural waters

被引：45

作者：

Campanella, L. ^{[1
]}

Lelo, D. ^{[1
]}

Martini, E. ^{[1
]}

Tomassetti, M. ^{[1
]}

机构：

[1] Univ Roma La Sapienza, Dept Chem, I-00185 Rome, Italy

来源：

ANALYTICA CHIMICA ACTA | 2007年 / 587卷 / 01期

关键词：

biosensor pesticide analysis; carbamate; organophosphorus; tyrosinase inhibition;

D O I：

10.1016/j.aca.2007.01.023

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Recent research performed in our laboratory (using 4 butyrylcholinesterase + choline oxidase enzyme electrode) suggested the validity of the biosensor approach using enzymes inhibition OPEEs (i.e. enzyme electrodes working in organic phase) in the case of organophosphorus and carbamate pesticides, which are poorly soluble in aqueous solutions. Since these pesticides are generally much more soluble in chloroform than in water, the present research aimed at analysing this class of pesticides using a tyrosinase inhibition OPEE operating in water-saturated chloroform medium. The tyrosinase biosensor was assembled using an oxygen amperometric transducer coupled to the tyrosinase enzyme, immobilized in kappa-carrageenan gel. Lastly a detailed comparison between the inhibition monoenzymatic tyrosinase and inhibition bienzymatic (butyrylcholinesterase + choline oxidase) OPEEs was performed and discussed in this work. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：22 / 32

页数：11

共 28 条

[1] Detection of organophosphorus insecticides with immobilized acetylcholinesterase - comparative study of two enzyme sensors [J].

Andreescu, S ;

Avramescu, A ;

Bala, C ;

Magearu, V ;

Marty, JL .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2002, 374 (01) :39-45

[2] Improved multianalyte detection of organophosphates and carbamates with disposable multielectrode biosensors using recombinant mutants of Drosophila acetylcholinesterase and artificial neural networks [J].

Bachmann, TT ;

Leca, B ;

Vilatte, F ;

Marty, JL ;

Fournier, D ;

Schmid, RD .

BIOSENSORS & BIOELECTRONICS, 2000, 15 (3-4) :193-201

[3] A BIOSENSOR AS WARNING DEVICE FOR THE DETECTION OF CYANIDE, CHLOROPHENOLS, ATRAZINE AND CARBAMATE PESTICIDES [J].

BESOMBES, JL ;

COSNIER, S ;

LABBE, P ;

REVERDY, G .

ANALYTICA CHIMICA ACTA, 1995, 311 (03) :255-263

[4] BUTYRYLCHOLINE ENZYME SENSOR FOR DETERMINING ORGANOPHOSPHORUS INHIBITORS [J].

CAMPANELLA, L ;

ACHILLI, M ;

SAMMARTINO, MP ;

TOMASSETTI, M .

BIOELECTROCHEMISTRY AND BIOENERGETICS, 1991, 26 (02) :237-249

[5] A NEW ENZYME-INHIBITION SENSOR FOR ORGANOPHOSPHORUS PESTICIDES ANALYSIS [J].

CAMPANELLA, L ;

COCCO, R ;

SAMMARTINO, MP ;

TOMASSETTI, M .

SCIENCE OF THE TOTAL ENVIRONMENT, 1992, 123 :1-16

[6] Monitoring the rancidification process in olive oils using a biosensor operating in organic solvents [J].

Campanella, L ;

Favero, G ;

Pastorino, M ;

Tomassetti, M .

BIOSENSORS & BIOELECTRONICS, 1999, 14 (02) :179-186

[7] Tyrosinase inhibition organic phase biosensor for triazinic and benzotriazinic pesticide analysis (part two) [J].

Campanella, L ;

Dragone, R ;

Lelo, D ;

Martini, E ;

Tomassetti, M .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2006, 384 (04) :915-921

[8] Determination of triazine pesticides using a new enzyme inhibition tyrosinase OPEE operating in chloroform [J].

Campanella, L ;

Bonanni, A ;

Martini, E ;

Todini, N ;

Tomassetti, M .

SENSORS AND ACTUATORS B-CHEMICAL, 2005, 111 :505-514

[9] A new organic phase bienzymatic electrode for lecithin analysis in food products [J].

Campanella, L ;

Pacifici, F ;

Sammartino, MP ;

Tomassetti, M .

BIOELECTROCHEMISTRY AND BIOENERGETICS, 1998, 47 (01) :25-38

[10] Organophosphorus pesticide (Paraoxon) analysis using solid state sensors [J].

Campanella, L ;

Colapicchioni, C ;

Favero, G ;

Sammartino, MP ;

Tomassetti, M .

SENSORS AND ACTUATORS B-CHEMICAL, 1996, 33 (1-3) :25-33

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