Microfluidic Device for Single-Molecule Experiments with Enhanced Photostability

被引：50

作者：

Lemke, Edward A. ^{[2
,3
]}

Gambin, Yann ^{[2
]}

Vandelinder, Virginia ^{[1
]}

Brustad, Eric M. ^{[3
]}

Liu, Hsiao-Wei ^{[2
]}

Schultz, Peter G. ^{[3
]}

Groisman, Alex ^{[1
]}

Deniz, Ashok A. ^{[2
]}

机构：

[1] Univ Calif San Diego, Dept Phys, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Dept Mol Biol, La Jolla, CA 92037 USA

[3] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2009年 / 131卷 / 38期

关键词：

FLUORESCENCE;

D O I：

10.1021/ja9027023

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A microfluidic device made of polydimethylsiloxane (PDMS) addresses key limitations in single-molecule fluorescence experiments by providing high dye photostability and low sample sticking. Photobleaching is dramatically reduced by deoxygenation via gas diffusion through porous channel watts. Rapid buffer exchange in a laminar sheath flow followed by optical interrogation minimizes surface-sample contacts and allows the in situ addition and combination of other reagents.

引用

页码：13610 / +

页数：4

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