Detection of Proteins in Serum by Micromagnetic Aptamer PCR (MAP) Technology

被引：90

作者：

Csordas, Andrew ^{[2
]}

Gerdon, Aren E. ^{[3
]}

Adams, Jonathan D. ^{[4
]}

Qian, Jiangrong ^{[5
]}

Oh, Seung Soo ^{[1
]}

Xiao, Yi ^{[1
]}

Soh, H. Tom ^{[1
]}

机构：

[1] Univ Calif Santa Barbara, Dept Mech Engn, Dept Mat, Santa Barbara, CA 93106 USA

[2] Univ Calif Santa Barbara, Inst Collaborat Biotechnol, Santa Barbara, CA 93106 USA

[3] Univ Cambridge Emmanuel Coll, Dept Chem, Boston, MA USA

[4] Univ Calif Santa Barbara, Dept Phys, Santa Barbara, CA 93106 USA

[5] Cynvenio Biosyst, Westlake Village, CA USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 02期

基金：

美国国家卫生研究院;

关键词：

analytical methods; aptamers; growth factors; polymerase chain reaction; proteins; ELECTROCHEMICAL DETECTION; IMMUNO-PCR; COMPLEX; MICROFLUIDICS; AMPLIFICATION; BIOMARKERS;

D O I：

10.1002/anie.200904846

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

"Chemical Equation Presented" On the MAP: The title technique combines microfluidic magnetic sample preparation with highly specific aptamerbased molecular recognition and quantitative PCR. Using platelet-derived growth factor as a model, a fM detection limit was obtained in fetal bovine serum with a dynamic range that spanned five orders of magnitude. © 2010 Wiley-VCH Verlag GmbH &. Co. KGaA.

引用

页码：355 / 358

页数：4

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