Selective isolation of live/dead cells using contactless dielectrophoresis (cDEP)

被引：245

作者：

Shafiee, Hadi ^{[1
,4
]}

Sano, Michael B. ^{[1
,2
,4
]}

Henslee, Erin A. ^{[2
,4
]}

Caldwell, John L. ^{[3
,4
]}

Davalos, Rafael V. ^{[1
,2
,4
]}

机构：

[1] Virginia Tech, Dept Engn Sci & Mech, Blacksburg, VA 24061 USA

[2] Wake Forest Univ, Virginia Tech, Sch Biomed Engn & Sci, Blacksburg, VA 24061 USA

[3] Virginia Tech, Bradley Dept Elect & Comp Engn, Blacksburg, VA 24061 USA

[4] Virginia Tech, ICTAS, Bioelectromech Syst Lab, Blacksburg, VA 24061 USA

来源：

LAB ON A CHIP | 2010年 / 10卷 / 04期

关键词：

CIRCULATING TUMOR-CELLS; PERIPHERAL-BLOOD; BREAST-CANCER; SEPARATION; BACTERIA; CHIP; MANIPULATION; DEVICES; SYSTEM; LIVE;

D O I：

10.1039/b920590j

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Contactless dielectrophoresis (cDEP) is a recently developed method of cell manipulation in which the electrodes are physically isolated from the sample. Here we present two microfluidic devices capable of selectively isolating live human leukemia cells from dead cells utilizing their electrical signatures. The effect of different voltages and frequencies on the gradient of the electric field and device performance was investigated numerically and validated experimentally. With these prototype devices we were able to achieve greater than 95% removal efficiency at 0.2-0.5 mm s(-1) with 100% selectivity between live and dead cells. In conjunction with enrichment, cDEP could be integrated with other technologies to yield fully automated lab-on-a-chip systems capable of sensing, sorting, and identifying rare cells.

引用

页码：438 / 445

页数：8

共 51 条

[1] Levitation and movement of human tumor cells using a printed circuit board device based on software-controlled dielectrophoresis [J].