Directed evolution: new parts and optimized function

被引：98

作者：

Dougherty, Michael J. ^{[1
]}

Arnold, Frances H. ^{[1
,2
]}

机构：

[1] CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA

[2] CALTECH, Div Biochem & Mol Biophys, Pasadena, CA 91125 USA

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2009年 / 20卷 / 04期

关键词：

TRANSCRIPTION FACTORS; SPECIFICITY; PROTEIN; EXPRESSION; DESIGN; GLYCOSYLTRANSFERASE; BIOSYNTHESIS; CONSTRUCTION; SYNTHASE; BIOLOGY;

D O I：

10.1016/j.copbio.2009.08.005

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Constructing novel biological systems that function in a robust and predictable manner requires better methods for discovering new functional molecules and for optimizing their assembly in novel biological contexts. By enabling functional diversification and optimization in the absence of detailed mechanistic understanding, directed evolution is a powerful complement to 'rational' engineering approaches. Aided by clever selection schemes, directed evolution has generated new parts for genetic circuits, cell-cell communication systems, and non-natural metabolic pathways in bacteria.

引用

页码：486 / 491

页数：6

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