Applying slow-release biocatalysis to the asymmetric reduction of ethyl 4-chloroacetoacetate

被引：25

作者：

Houng, JY ^{[1
]}

Liau, JS

机构：

[1] I Shou Univ, Dept Chem Engn, Kaohsiung, Taiwan

[2] Natl Tsing Hua Univ, Dept Chem Engn, Hsinchu, Taiwan

来源：

BIOTECHNOLOGY LETTERS | 2003年 / 25卷 / 01期

关键词：

absorbing resins; asymmetric reduction; baker's yeast; stereoselectivity; substrate inhibition;

D O I：

10.1023/A:1021761627090

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Amberlite XAD 2 resin enhanced the asymmetric reduction of ethyl 4-chloroacetoacetate (ECA) to S-4-chloro-3-hydroxybutyric acid ethyl ester as catalyzed by Saccharomyces cerevisiae. The absorbed ECA was released slowly to the solution during the reaction so that the substrate inhibition and the spontaneous chemical hydrolysis of ECA were considerably lessened. With 75 g resin l(-1) and ECA at 74 mM, the reaction yield and the product's optical purity increased from 75% to 84% and from 88% to 93%, respectively.

引用

页码：17 / 21

页数：5

共 15 条

[1] APPLICATION OF A PRACTICAL BIOCATALYTIC REDUCTION TO AN ENANTIOSELECTIVE SYNTHESIS OF THE 5H-2,3-BENZODIAZEPINE LY300164 [J].