Evaluation of silicon dioxide-based coating enriched with bioactive peptides mapped on human vitronectin and fibronectin:: in vitro and in vivo assays

被引:14
作者
Dettin, Monica
Bagno, Andrea [1 ]
Morpurgo, Margherita
Cacchioli, Antonio
Conconi, Maria Teresa
Di Bello, Carlo
Gabbi, Carlo
Gambaretto, Roberta
Parnigotto, Pier Paolo
Pizzinato, Sara
Ravanetti, Francesca
Guglielmi, Massimo
机构
[1] Univ Padua, Dept Chem Proc Engn, Padua, Italy
[2] Univ Padua, Dept Pharmaceut Sci, Padua, Italy
[3] Univ Padua, Dept Mech Engn, Padua, Italy
[4] Univ Parma, Dept Anim Hlth, I-43100 Parma, Italy
来源
TISSUE ENGINEERING | 2006年 / 12卷 / 12期
关键词
D O I
10.1089/ten.2006.12.3509
中图分类号
Q813 [细胞工程];
学科分类号
摘要
A wide range of biochemical signals promoting cell functions ( adhesion, migration, proliferation, and differentiation) and thereby improving the osseointegration process are currently investigated. Unfortunately, their application for the production of bioactive implantable devices is often hampered by their insolubility; instability; and limited availability of a large amount of inexpensive, high-purity samples. An attractive alternative is the use of short peptides carrying the minimum active sequence of the natural factors. Synthetic peptides mapped on fibronectin and vitronectin have been demonstrated to enhance cell adhesion both to polystyrene and acellular bone matrix; in particular, a nonapeptide sequence from human vitronectin works via an osteoblast-specific adhesion mechanism. In this study, we incorporated these peptides into a sol-gel silica dressing applied to coat sand-blasted and acid-attacked titanium samples; measured the kinetic of peptide release; and used titanium disks, coated with a peptide-enriched film, as substrates to determine the peptide concentration that maximizes cell adhesion in vitro. We also evaluated in vivo the capacity of the vitronectin-derived peptide to improve osteogenic activity: histologic analysis revealed markedly improved osteogenic activity around peptide-enriched samples. This article also discusses the role of surface characteristics and the importance of bioactive peptides.
引用
收藏
页码:3509 / 3523
页数:15
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