Separation of proteins using a novel two-depth miniaturized free-flow electrophoresis device with multiple outlet fractionation channels

被引：17

作者：

Becker, Marco ^{[1
]}

Marggraf, Ulrich ^{[1
]}

Janasek, Dirk ^{[1
]}

机构：

[1] ISAS Inst Analyt Sci, D-44139 Dortmund, Germany

来源：

JOURNAL OF CHROMATOGRAPHY A | 2009年 / 1216卷 / 47期

关键词：

Microfluidic; Free-flow electrophoresis; Microfabrication; CHIP;

D O I：

10.1016/j.chroma.2009.06.079

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel free-flow electrophoresis glass chip design with two-depth etched structures for the separation and fractionation of proteins is presented. The microfluidic structures etched in two depths enhance the flow characteristics inside the miniaturized device. A novel nine-port outlet interface enables the fractionation of the separated analytes. The separation and focussing of a protein sample Mixture demonstrated the ability of the new chip. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：8265 / 8269

页数：5

共 24 条

[21] Temperature gradient focusing for microchannel separations [J].