Libraries of composite polyfluors built from fluorescent deoxyribosides

被引：108

作者：

Gao, JM

Strässler, C

Tahmassebi, D

Kool, ET ^{[1
]}

机构：

[1] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

[2] Univ San Diego, Dept Chem, San Diego, CA 92110 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2002年 / 124卷 / 39期

关键词：

D O I：

10.1021/ja027197a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report on a new class of water-soluble fluorescent molecules (polyfluors) that are composed of multiple individual fluorophores assembled on a DNA-like backbone. Four fluorophore deoxyribosides were synthesized, and these individual molecules were assembled into oligofluor strings on a DNA synthesizer. A library of 256 tetrafluors was generated by split and pool methods on polystyrene beads. Images of the library under a fluorescence microscope revealed at least 40-50 different hues and intensities. Selected tetrafluors were resynthesized in pure form in solution and displayed properties, such as large Stokes shifts, that individual fluorophores do not have. Copyright © 2002 American Chemical Society.

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页码：11590 / 11591

页数：2

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