Sperm tsRNAs contribute to intergenerational inheritance of an acquired metabolic disorder

被引：1062

作者：

Chen, Qi ^{[1
,3
]}

Yan, Menghong ^{[2
]}

Cao, Zhonghong ^{[1
,5
]}

Li, Xin ^{[1
]}

Zhang, Yunfang ^{[1
,5
]}

Shi, Junchao ^{[1
,5
]}

Feng, Gui-hai ^{[1
]}

Peng, Hongying ^{[1
,4
]}

Zhang, Xudong ^{[1
,5
]}

Zhang, Ying ^{[1
]}

Qian, Jingjing ^{[1
,5
]}

Duan, Enkui ^{[1
]}

Zhai, Qiwei ^{[2
]}

Zhou, Qi ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Zool, State Key Lab Stem Cell & Reprod Biol, Beijing 100101, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Biol Sci, Ctr Excellence Mol Cell Sci, Key Lab Nutr & Metab, Shanghai 200031, Peoples R China

[3] Univ Nevada, Sch Med, Dept Physiol & Cell Biol, Reno, NV 89512 USA

[4] Beijing Univ Chinese Med, Beijing Royal Integrat Med Hosp, Beijing, Peoples R China

[5] Univ Chinese Acad Sci, Beijing 100049, Peoples R China

来源：

SCIENCE | 2016年 / 351卷 / 6271期

基金：

中国国家自然科学基金;

关键词：

INDUCED TRANSGENERATIONAL INHERITANCE; SMALL RNAS; PARAMUTATION; RAT;

D O I：

10.1126/science.aad7977

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Increasing evidence indicates that metabolic disorders in offspring can result from the father's diet, but the mechanism remains unclear. In a paternal mouse model given a high-fat diet (HFD), we showed that a subset of sperm transfer RNA-derived small RNAs (tsRNAs), mainly from 5' transfer RNA halves and ranging in size from 30 to 34 nucleotides, exhibited changes in expression profiles and RNA modifications. Injection of sperm tsRNA fractions from HFD males into normal zygotes generated metabolic disorders in the F-1 offspring and altered gene expression of metabolic pathways in early embryos and islets of F-1 offspring, which was unrelated to DNA methylation at CpG-enriched regions. Hence, sperm tsRNAs represent a paternal epigenetic factor that may mediate intergenerational inheritance of diet-induced metabolic disorders.

引用

页码：397 / 400

页数：4

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