Denaturation processes in gamma irradiated proteins studied by differential scanning calorimetry

被引：33

作者：

Ciesla, K

Roos, YH

Gluszewski, W

机构：

[1] Inst Nucl Chem & Technol, PL-03195 Warsaw, Poland

[2] Univ Helsinki, Dept Food Technol, FIN-00014 Helsinki, Finland

来源：

RADIATION PHYSICS AND CHEMISTRY | 2000年 / 58卷 / 03期

关键词：

globular proteins; gamma irradiation; denaturation; differential scanning calorimetry;

D O I：

10.1016/S0969-806X(99)00380-1

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Differential scanning calorimetry was applied to study the influence of gamma irradiation on the process of denaturation of proteins, occurring in water suspensions during heating. Irradiations of solid native samples and of water suspensions of the selected globular proteins were performed in air atmosphere at ambient temperature or in solid CO2, by applying 20-30 kGy, 3 kGy and 2.5 kGy doses. A decrease of peak and onset temperatures, broadening of the denaturation endothermal effects, as well as a decline in denaturation enthalpy was observed as a result of irradiation. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：233 / 243

页数：11

共 23 条

[1] RADIATION DAMAGE TO CYTOCHROME C AND HEMOGLOBIN [J].