Diversity of Clostridium difficile in pigs and other animals in Slovenia

被引：91

作者：

Avbersek, Jana ^{[1
]}

Janezic, Sandra ^{[2
]}

Pate, Mateja ^{[1
]}

Rupnik, Maja ^{[2
,3
]}

Zidaric, Valerija ^{[2
]}

Logar, Katarina ^{[1
]}

Vengust, Modest ^{[1
]}

Zemljic, Mateja ^{[2
]}

Pirs, Tina ^{[1
]}

Ocepek, Matjaz ^{[1
]}

机构：

[1] Univ Ljubljana, Fac Vet, SI-1000 Ljubljana, Slovenia

[2] Inst Publ Hlth Maribor, SI-1000 Maribor, Slovenia

[3] Univ Maribor, Fac Med, SI-2000 Maribor, Slovenia

来源：

ANAEROBE | 2009年 / 15卷 / 06期

关键词：

Clostridium difficile; Animals; PFGE; Toxinotyping; PCR-ribotyping; FIELD GEL-ELECTROPHORESIS; PCR RIBOTYPES; PREVALENCE; INFECTION; STRAINS; HUMANS; HORSES; CALVES; TOXIN; MEAT;

D O I：

10.1016/j.anaerobe.2009.07.004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 [微生物学];

摘要：

A study of Clostridium difficile diversity in pigs, calves and horses in Slovenia was conducted. A total of 547 samples were collected and C difficile was isolated from 247/485 (50.9%) piglet samples, from 4/42 (9.5%) calf samples, and 1/20 (5%) horse samples. The isolates were characterized by toxinotyping, PCR-ribotyping, and pulsed-field gel electrophoresis (PFGE) using restriction endonuclease Smal. Piglet isolates belonged to two toxinotypes (V and 0), four PCR-ribotypes (066, 029, SI 011, SI 010), and six pulsotypes. Bovine isolates were grouped into two toxinotypes (XIa and 0), three PCR-ribotypes (077, 002, 033), and three pulsotypes. The only equine isolate was indistinguishable from one calf isolate (XIa/033) in toxinotype, PCR-ribotype, and pulsotype. None of detected genotypes was present in all three animal hosts. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：252 / 255

页数：4

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