A triaxial probe for on-line proteolysis coupled with hydrogen/deuterium exchange-electrospray mass spectrometry

被引：11

作者：

Chen, Maolian

Cook, Kelsey D. ^{[1
]}

Kheterpal, Indu

Wetzel, Ronald

机构：

[1] Univ Tennessee, Dept Chem, Knoxville, TN 37996 USA

[2] Louisiana State Univ, Ctr Biomodular MultiScale Syst, Baton Rouge, LA 70803 USA

[3] Univ Tennessee, Grad Sch Med, Knoxville, TN 37996 USA

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 2007年 / 18卷 / 02期

关键词：

D O I：

10.1016/j.jasms.2006.09.011

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

on-line proteolysis system utilizing a triaxial electrospray probe was developed to aid localization of the hydrogen-bonding interaction sites in hydrogen/ deuterium exchange-mass spectrometry (HDX-MS) studies of A beta (1-40) fibrils. The probe allows delayed introduction of the organic solvent component needed for stable electrospray, thus enhancing hydrolysis performance relative to that of a coaxial probe. Effective on-line digestion was accomplished in similar to 12 s. The probe should be of general utility for HDX-MS studies of amyloid fibrils and other protein aggregates. (c) 2007 American Society for Mass Spectrometry.

引用

页码：208 / 217

页数：10

共 41 条

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