Engineered fluorescent proteins: innovations and applications

被引：85

作者：

Davidson, Michael W. ^{[1
,2
]}

Campbell, Robert E. ^{[3
]}

机构：

[1] Florida State Univ, Natl High Magnet Field Lab, Tallahassee, FL 32306 USA

[2] Florida State Univ, Dept Biol Sci, Tallahassee, FL 32306 USA

[3] Univ Alberta, Dept Chem, Edmonton, AB, Canada

来源：

NATURE METHODS | 2009年 / 6卷 / 10期

关键词：

MONOMERIC RED; BRIGHT; ORANGE; PHOTOCONVERSION; PHOTOSTABILITY;

D O I：

10.1038/nmeth1009-713

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Despite expansion of the fluorescent protein and optical highlighter palette into the orange to far-red range of the visible spectrum, achieving performance equivalent to that of EGFP has continued to elude protein engineers.

引用

页码：713 / 717

页数：5

共 19 条

[1] Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors [J].

Ai, Hui-wang ;

Hazelwood, Kristin L. ;

Davidson, Michael W. ;

Campbell, Robert E. .

NATURE METHODS, 2008, 5 (05) :401-403

[2] mRuby, a Bright Monomeric Red Fluorescent Protein for Labeling of Subcellular Structures [J].

Kredel, Simone ;

Oswald, Franz ;

Nienhaus, Karin ;

Deuschle, Karen ;

Roecker, Carlheinz ;

Wolff, Michael ;

Heilker, Ralf ;

Nienhaus, G. Ulrich ;

Wiedenmann, Joerg .

PLOS ONE, 2009, 4 (02)

[3]

Kremers GJ, 2009, NAT METHODS, V6, P355, DOI [10.1038/NMETH.1319, 10.1038/nmeth.1319]

[4] Fluorescent proteins from nonbioluminescent Anthozoa species [J].

Matz, MV ;

Fradkov, AF ;

Labas, YA ;

Savitsky, AP ;

Zaraisky, AG ;

Markelov, ML ;

Lukyanov, SA .

NATURE BIOTECHNOLOGY, 1999, 17 (10) :969-973

[5]

McKinney SA, 2009, NAT METHODS, V6, P131, DOI [10.1038/nmeth.1296, 10.1038/NMETH.1296]

[6] Bright monomeric red fluorescent protein with an extended fluorescence lifetime [J].

Merzlyak, Ekaterina M. ;

Goedhart, Joachim ;

Shcherbo, Dmitry ;

Bulina, Mariya E. ;

Shcheglov, Aleksandr S. ;

Fradkov, Arkady F. ;

Gaintzeva, Anna ;

Lukyanov, Konstantin A. ;

Lukyanov, Sergey ;

Gadella, Theodorus W. J. ;

Chudakov, Dmitriy M. .

NATURE METHODS, 2007, 4 (07) :555-557

[7] A photoactivatable GFP for selective photolabeling of proteins and cells [J].

Patterson, GH ;

Lippincott-Schwartz, J .

SCIENCE, 2002, 297 (5588) :1873-1877

[8] Improving the photostability of bright monomeric orange and red fluorescent proteins [J].

Shaner, Nathan C. ;

Lin, Michael Z. ;

McKeown, Michael R. ;

Steinbach, Paul A. ;

Hazelwood, Kristin L. ;

Davidson, Michael W. ;

Tsien, Roger Y. .

NATURE METHODS, 2008, 5 (06) :545-551

[9] Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp red fluorescent protein [J].

Shaner, NC ;

Campbell, RE ;

Steinbach, PA ;

Giepmans, BNG ;

Palmer, AE ;

Tsien, RY .

NATURE BIOTECHNOLOGY, 2004, 22 (12) :1567-1572

[10] A guide to choosing fluorescent proteins [J].

Shaner, NC ;

Steinbach, PA ;

Tsien, RY .

NATURE METHODS, 2005, 2 (12) :905-909

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