Cationic Glyconanoparticles: Their Complexation with DNA, Cellular Uptake, and Transfection Efficiencies

被引：53

作者：

Ahmed, Marya ^{[1
,2
]}

Deng, Zhicheng ^{[1
,2
]}

Liu, Shiyong ^{[3
]}

Lafrenie, Robert ^{[4
]}

Kumar, Aseem ^{[2
]}

Narain, Ravin ^{[1
,2
]}

机构：

[1] Univ Alberta, Dept Chem & Mat Engn, Edmonton, AB T6G 2G6, Canada

[2] Laurentian Univ, Dept Chem & Biochem, Biomol Sci Program, Sudbury, ON P3E 2C6, Canada

[3] Univ Sci & Technol China, Dept Polymer Sci & Engn, Hefei 230026, Anhui, Peoples R China

[4] Reg Canc Program Hosp, Sudbury, ON, Canada

来源：

BIOCONJUGATE CHEMISTRY | 2009年 / 20卷 / 11期

基金：

加拿大自然科学与工程研究理事会;

关键词：

GOLD NANOPARTICLES; GENE-DELIVERY; POLYMER; CHITOSAN; VECTORS; BINDING; ABILITY; SIZE;

D O I：

10.1021/bc900350c

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

There is a need to synthesize new gene delivery vehicles that can deal with the problems of endosomal escape and nuclear entry. We propose cationic glycopolymer-stabilized gold nanoparticles as an effective gene delivery system. The cationic glyconanoparticles synthesized were revealed to be biocompatible and arc resistant to aggregation in physiological conditions. The complexation of DNA to the cationic glyconanoparticles is determined by agarose gel electrophoresis. The localization of the DNA-glyconanoparticles inside the Hela cell line and their mechanism of uptake is studied by confocal microscopy. Finally, the efficacy of the glyconanoparticles as gene delivery vehicles in vitro is studied by their complexation with cyanine fluorescence protein encoded plasmid, and the transfection efficiency is found to be comparable to the commercially available control Lipofectamine 2000.

引用

页码：2169 / 2176

页数：8

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