Analysis of aPKCλ and aPKCC reveals multiple and redundant functions during vertebrate retinogenesis

被引:39
作者
Cui, Shuang
Otten, Cecile
Rohr, Stefan
Abdelilah-Seyfried, Salim
Link, Brian A. [1 ]
机构
[1] Med Coll Wisconsin, Dept Cell Biol Neurobiol & Anat, Milwaukee, WI 53226 USA
[2] Max Delbruck Ctr Mol Med, D-13125 Berlin, Germany
关键词
neurogenesis; RPE; polarity; migration; ocular development; morphogenesis;
D O I
10.1016/j.mcn.2006.11.016
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Retinal lamination is known to depend on cell polarity and localized signaling. In vertebrates, atypical protein kinase C proteins, aPKC lambda/iota and aPKC zeta, are essential for apical-basal cell polarity. However, it is not known to what extent functional redundancy has precluded a comprehensive functional characterization of aPKC signaling during vertebrate retinogenesis. Here, we show that aPKCs lambda and zeta are functionally redundant for multiple aspects of retinogenesis including mitotic division location and orientation, cell-type positioning, and retinal pigment epithelial (RPE) and photoreceptor cell morphogenesis. Genetic mosaic analyses demonstrate a cell-autonomous requirement of aPKCs for RPE and photoreceptor development, and a cell-non-cell-autonomous function that is intrinsic to the neural retina for cell-type positioning. Our observations uncover a previously unappreciated involvement of aPKCC during zebrafish retinogenesis and suggest that aPKC signaling primes the retinal environment for appropriate cell migration of post-mitotic progenitor cells but is not essential for correct cell-type specification. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码:431 / 444
页数:14
相关论文
共 51 条
[21]   Function of atypical protein kinase C λ in differentiating photoreceptors is required for proper lamination of mouse retina [J].
Koike, C ;
Nishida, A ;
Akimoto, K ;
Nakaya, MA ;
Noda, T ;
Ohno, S ;
Furukawa, T .
JOURNAL OF NEUROSCIENCE, 2005, 25 (44) :10290-10298
[22]   Photoreceptor cells in flies and mammals: Crumby homology? [J].
Kowalczyk, AP ;
Moses, K .
DEVELOPMENTAL CELL, 2002, 2 (03) :253-254
[23]   Inductive effects of the retinal pigmented epithelium (RPE) on histogenesis of the avian retina as revealed by retinospheroid technology [J].
Layer, PG ;
Rothermel, A ;
Willbold, E .
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 1998, 9 (03) :257-262
[24]   Targeted disruption of the ζPKC gene results in the impairment of the NF-κB pathway [J].
Leitges, M ;
Sanz, L ;
Martin, P ;
Duran, A ;
Braun, U ;
García, JF ;
Camacho, F ;
Diaz-Meco, MT ;
Rennert, PD ;
Moscat, J .
MOLECULAR CELL, 2001, 8 (04) :771-780
[25]  
Link BA, 2000, DEVELOPMENT, V127, P2177
[26]   Evidence for directed mitotic cleavage plane reorientations during retinal development within the zebrafish [J].
Link, BA .
BIOLOGICAL BULLETIN, 2001, 201 (02) :254-255
[27]   Vertebrate neural cell-fate determination: Lessons from the retina [J].
Livesey, FJ ;
Cepko, CL .
NATURE REVIEWS NEUROSCIENCE, 2001, 2 (02) :109-118
[28]  
Malicki J, 1999, DEVELOPMENT, V126, P1235
[29]   Regulated protein-protein interaction between aPKC and PAR-3 plays an essential role in the polarization of epithelial cells [J].
Nagai-Tamai, Y ;
Mizuno, K ;
Hirose, T ;
Suzuki, A ;
Ohno, S .
GENES TO CELLS, 2002, 7 (11) :1161-1171
[30]  
OTMORI Y, 2006, CURR BIOL, V16, P945