Bovine colostrum in oral treatment of enterogenic endotoxaemia in rats

被引:28
作者
Döhler, JR
Nebermann, L
机构
[1] Klinikum Plau See, Dept Orthopaed Trauma & Hand Surg, Mecklenburg Vorpommern, Germany
[2] German Navy, Wilhelmshaven, Germany
来源
CRITICAL CARE | 2002年 / 6卷 / 06期
关键词
colostrum; endotoxaemia; experimental septic shock;
D O I
10.1186/cc1819
中图分类号
R4 [临床医学];
学科分类号
1002 ; 100602 ;
摘要
Introduction Under conditions of shock, bacteria and endotoxins in the intestines can traverse the mucosal barrier by translocation and enter the blood and lymphatic system. Immunoglobulins and lactoferrin have been reported to neutralize endotoxins and bacteria. We studied the essential therapeutic factors of colostrum products in an animal experiment. Method We simulated endotoxaemia by per-oral administration of a suspension of Escherichia coli and antibiotics into the duodenum of anaesthetized rats after giving intraperitoneal carrageenan. At the same time, pure bovine colostrum or lactoferrin-enriched bovine colostrum was given. Therapeutic effects were studied by examining plasma endotoxin activity and bacterial contamination of mesenterial lymph nodes and peritoneal lavages. Albumin was used in a control group. Results The most effective bovine colostrum was able to reduce the maximum plasma endotoxin value by 67% as compared with the albumin group. The combination of this colostrum with. lactoferrin brought about a reduction by 80%. The reduction in bacterial contamination of lymph nodes and peritoneal lavages; was also evident. Conclusion Both gammaglobulin and lactoferrin may help to eliminate endotoxins when bovine colostrum is administered into the gut in conditions of septic shock.
引用
收藏
页码:536 / 539
页数:4
相关论文
共 17 条
[1]   LACTOFERRIN IS A LIPID A-BINDING PROTEIN [J].
APPELMELK, BJ ;
AN, YQ ;
GEERTS, M ;
THIJS, BG ;
DEBOER, HA ;
MACLAREN, D ;
DEGRAAFF, J ;
NUIJENS, JH .
INFECTION AND IMMUNITY, 1994, 62 (06) :2628-2632
[2]   FIELD TRIAL OF AN INFANT FORMULA CONTAINING ANTIROTAVIRUS AND ANTI-ESCHERICHIA-COLI MILK ANTIBODIES FROM HYPERIMMUNIZED COWS [J].
BRUNSER, O ;
ESPINOZA, J ;
FIGUEROA, G ;
ARAYA, M ;
SPENCER, E ;
HILPERT, H ;
LINKAMSTER, H ;
BRUSSOW, H .
JOURNAL OF PEDIATRIC GASTROENTEROLOGY AND NUTRITION, 1992, 15 (01) :63-72
[3]  
BURKE DJ, 1989, ARCH SURG-CHICAGO, V124, P1396
[4]   Escherichia coli O157:H7 causes more-severe systemic disease in suckling piglets than in colostrum-deprived neonatal piglets [J].
Dean-Nystrom, EA ;
Pohlenz, JFL ;
Moon, HW ;
O'Brien, AD .
INFECTION AND IMMUNITY, 2000, 68 (04) :2356-2358
[5]   EFFECT OF HEMORRHAGIC-SHOCK ON BACTERIAL TRANSLOCATION, INTESTINAL MORPHOLOGY, AND INTESTINAL PERMEABILITY IN CONVENTIONAL AND ANTIBIOTIC-DECONTAMINATED RATS [J].
DEITCH, EA ;
MORRISON, J ;
BERG, R ;
SPECIAN, RD .
CRITICAL CARE MEDICINE, 1990, 18 (05) :529-536
[6]   Anti-endotoxin antibodies in human milk: correlation with infection of the newborn [J].
Feist, N ;
Berger, D ;
Speer, CP .
ACTA PAEDIATRICA, 2000, 89 (09) :1087-1092
[7]   AN IMPROVED CHROMOGENIC SUBSTRATE ENDOTOXIN ASSAY FOR CLINICAL USE [J].
HARRIS, RI ;
STONE, PCW ;
STUART, J .
JOURNAL OF CLINICAL PATHOLOGY, 1983, 36 (10) :1145-1149
[8]   Inhibitory effects of bovine lactoferrin on the adherence of enterotoxigenic Escherichia coli to host cells [J].
Kawasaki, Y ;
Tazume, S ;
Shimizu, K ;
Matsuzawa, H ;
Dosako, S ;
Isoda, H ;
Tsukiji, M ;
Fujimura, R ;
Muranaka, Y ;
Isihida, H .
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2000, 64 (02) :348-354
[9]   A standard immunoglobulin preparation produced from bovine colostra shows antibody reactivity and neutralization activity against Shiga-like toxins and EHEC-hemolysin of Escherichia coli O157:H7 [J].
Lissner, R ;
Schmidt, H ;
Karch, H .
INFECTION, 1996, 24 (05) :378-383
[10]   Measurement of endotoxin activity in critically ill patients using whole blood neutrophil dependent chemiluminescence [J].
Marshall, JC ;
Walker, PM ;
Foster, DM ;
Harris, D ;
Ribeiro, M ;
Paice, J ;
Romaschin, AD ;
Derzko, AN .
CRITICAL CARE, 2002, 6 (04) :342-348