Photon-counting distribution of fluorescence from a blinking molecule

被引：22

作者：

Molski, A ^{[1
]}

机构：

[1] Adam Mickiewicz Univ, Dept Phys Chem, PL-60780 Poznan, Poland

来源：

CHEMICAL PHYSICS LETTERS | 2000年 / 324卷 / 04期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/S0009-2614(00)00595-9

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Photon-counting distribution of fluorescence from a single molecule is modeled as a doubly stochastic Poisson distribution. A two-state model is considered where fluorescence from a dye molecule jumps between an 'on' (= high) intensity level and an 'off' (= low, background) level. Those intensity jumps may be caused by sojourns of a molecule into a non-emissive triplet state. Illustrative examples of photon-counting distribution are calculated for three parameter regimes. In the slow blinking regime, the 'on' and 'off' lifetimes are much longer then the integration time (= bin time). In the intermediate regime at least one of the 'on' and 'off' Lifetimes is comparable with the integration time. In the fast blinking regime, the 'on' and 'off' lifetimes are shorter then the integration time. The photon-counting distribution can be helpful in establishing whether an experimental intensity histogram is consistent with triplet blinking. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：301 / 306

页数：6

共 12 条

[1] Theory of single-molecule fluorescence spectroscopy of two-state systems [J].

Berezhkovskii, AM ;

Szabo, A ;

Weiss, GH .

JOURNAL OF CHEMICAL PHYSICS, 1999, 110 (18) :9145-9150

[2]

Cox D. R., 1980, POINT PROCESSES

[3] Quantum jumps of single molecules at room temperature [J].

Ha, T ;

Enderle, T ;

Chemla, DS ;

Selvin, PR ;

Weiss, S .

CHEMICAL PHYSICS LETTERS, 1997, 271 (1-3) :1-5

[4] Illuminating single molecules in condensed matter [J].

Moerner, WE ;

Orrit, M .

SCIENCE, 1999, 283 (5408) :1670-+

[5] Theory of time-resolved single-molecule fluorescence spectroscopy [J].

Molski, A ;

Hofkens, J ;

Gensch, T ;

Boens, N ;

De Schryver, F .

CHEMICAL PHYSICS LETTERS, 2000, 318 (4-5) :325-332

[6] Optical detection of single molecules [J].

Nie, SM ;

Zare, RN .

ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE, 1997, 26 :567-596

[7] Statistical analyses and theoretical models of single-molecule enzymatic dynamics [J].

Schenter, GK ;

Lu, HP ;

Xie, XS .

JOURNAL OF PHYSICAL CHEMISTRY A, 1999, 103 (49) :10477-10488

[8] Time-varying triplet state lifetimes of single molecules [J].

Veerman, JA ;

Garcia-Parajo, MF ;

Kuipers, L ;

van Hulst, NF .

PHYSICAL REVIEW LETTERS, 1999, 83 (11) :2155-2158

[9] Fluorescence spectroscopy of single biomolecules [J].

Weiss, S .

SCIENCE, 1999, 283 (5408) :1676-1683

[10] Single-molecule detection fluorescence of surface-bound species in vacuum [J].

Weston, KD ;

Carson, PJ ;

DeAro, JA ;

Buratto, SK .

CHEMICAL PHYSICS LETTERS, 1999, 308 (1-2) :58-64

← 1 2 →