Regulation and trafficking of three distinct 18 S ribosomal RNAs during development of the malaria parasite

被引:84
作者
Li, J
Gutell, RR
Damberger, SH
Wirtz, RA
Kissinger, JC
Rogers, MJ
Sattabongkot, J
McCutchan, TF
机构
[1] NIAID,GROWTH & DEV SECT,PARASIT DIS LAB,NIH,BETHESDA,MD 20892
[2] UNIV COLORADO,DEPT MCD BIOL,BOULDER,CO 80309
[3] UNIV COLORADO,DEPT CHEM & BIOCHEM,BOULDER,CO 80309
[4] CTR DIS CONTROL & PREVENT,DIV PARASIT DIS,ENTOMOL BRANCH,ATLANTA,GA 30341
[5] USA,MED COMPONENT,DEPT ENTOMOL,BANGKOK,THAILAND
关键词
small subunit (SSU) rRNA; ribosome; development; malaria; translational control;
D O I
10.1006/jmbi.1997.1038
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The human malaria parasite Plasmodium vivax has been shown to regulate the transcription of two distinct 18 RNAs during development. Here we show a third and distinctive type of ribosome that is present shortly after zygote formation, a transcriptional pattern of ribosome types that relates closely to the developmental state of the parasite and a phenomenon that separates ribosomal types at a critical phase of maturation. The A-type ribosome is predominantly found in infected erythrocytes of the vertebrate and the mosquito blood meal. Transcripts from the A gene are replaced by transcripts from another locus, the O gene, shortly after fertilization and increase in number as the parasite develops on the mosquito midgut. Transcripts from another locus, the S gene, begins as the oocyst form of the parasite matures. RNA transcripts from the S gene are preferentially included in sporozoites that bud off from the oocyst and migrate to the salivary gland while the O gene transcripts are left within the oocyst. Although all three genes are typically eukaryotic in structure, the O gene transcript, described here, varies from the other two in core regions of the rRNA that are involved in mRNA decoding and translational termination. We now can correlate developmental progression of the parasite with changes in regions of rRNA sequence that are broadly conserved, where sequence alterations have been related to function in other systems and whose effects can be studied outside of Plasm odium. This should allow assessment of the role of translational control in parasite development. (C) 1997 Academic Press Limited.
引用
收藏
页码:203 / 213
页数:11
相关论文
共 33 条
[11]   TRANSITION OF PLASMODIUM-VIVAX RIBOSOME TYPES CORRESPONDS TO SPOROZOITE DIFFERENTIATION IN THE MOSQUITO [J].
LI, J ;
WIRTZ, RA ;
MCCONKEY, GA ;
SATTABONGKOT, J ;
MCCUTCHAN, TF .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1994, 65 (02) :283-289
[12]   PLASMODIUM-BERGHEI - QUANTITATION OF INVITRO EFFECTS OF ANTIMALARIAL-DRUGS ON EXOERYTHROCYTIC DEVELOPMENT BY A RIBOSOMAL-RNA PROBE [J].
LI, J ;
ZHU, JD ;
APPIAH, A ;
MCCUTCHAN, TF ;
LONG, GW ;
MILHOUS, WK ;
HOLLINGDALE, MR .
EXPERIMENTAL PARASITOLOGY, 1991, 72 (04) :450-458
[13]   PLASMODIUM - GENUS-CONSERVED PRIMERS FOR SPECIES IDENTIFICATION AND QUANTITATION [J].
LI, J ;
WIRTZ, RA ;
MCCONKEY, GA ;
SATTABONGKOT, J ;
WATERS, AP ;
ROGERS, MJ ;
MCCUTCHAN, TF .
EXPERIMENTAL PARASITOLOGY, 1995, 81 (02) :182-190
[14]  
LI J, 1997, IN PRESS AM J TROP M
[15]  
LI WH, 1991, FUNDAMENTALS MOL EVO, P136
[16]   The accuracy center of a eukaryotic ribosome [J].
Liebman, SW ;
Chernoff, YO ;
Liu, R .
BIOCHEMISTRY AND CELL BIOLOGY, 1995, 73 (11-12) :1141-1149
[17]   GENETIC AND COMPARATIVE ANALYSES REVEAL AN ALTERNATIVE SECONDARY STRUCTURE IN THE REGION OF NT-912 OF ESCHERICHIA-COLI 16S RIBOSOMAL-RNA [J].
LODMELL, JS ;
GUTELL, RR ;
DAHLBERG, AE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (23) :10555-10559
[18]  
MCCUTCHAN TF, 1986, INT REV CYTOL, V99, P295
[19]   PRIMARY SEQUENCES OF 2 SMALL SUBUNIT RIBOSOMAL-RNA GENES FROM PLASMODIUM-FALCIPARUM [J].
MCCUTCHAN, TF ;
DELACRUZ, VF ;
LAL, AA ;
GUNDERSON, JH ;
ELWOOD, HJ ;
SOGIN, ML .
MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1988, 28 (01) :63-68
[20]   THE CYTOPLASMIC RIBOSOMAL-RNAS OF PLASMODIUM SPP [J].
MCCUTCHAN, TF ;
LI, J ;
MCCONKEY, GA ;
ROGERS, MJ ;
WATERS, AP .
PARASITOLOGY TODAY, 1995, 11 (04) :134-138