RNAi: gene-silencing in therapeutic intervention

被引：114

作者：

Shuey, DJ ^{[1
]}

McCallus, DE ^{[1
]}

Giordano, T ^{[1
]}

机构：

[1] Nucleon, Malvern, PA 19355 USA

来源：

DRUG DISCOVERY TODAY | 2002年 / 7卷 / 20期

关键词：

D O I：

10.1016/S1359-6446(02)02474-1

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Several rapidly developing RNA interference (RNAi) methodologies hold the promise to selectively inhibit gene expression in mammals. RNAi is an innate cellular process activated when a double-stranded RNA (dsRNA) molecule of greater than 19 duplex nucleotides enters the cell, causing the degradation of not only the invading dsRNA molecule, but also single-stranded (ssRNAs) RNAs of identical sequences, including endogenous mRNAs. As such, RNAi technology is currently being evaluated not only as an extremely powerful instrument for functional genomic analyses, but also as a potentially useful method to develop highly specific dsRNA based gene-silencing therapeutics.

引用

页码：1040 / 1046

页数：7

共 53 条

[41] Stable suppression of gene expression by RNAi in mammalian cells
Paddison, PJ
Caudy, AA
Hannon, GJ
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (03) : 1443 - 1448
[42] Systemic acquired silencing: Transgene-specific post-transcriptional silencing is transmitted by grafting from silenced stocks to non-silenced scions
Palauqui, JC
Elmayan, T
Pollien, JM
Vaucheret, H
[J]. EMBO JOURNAL, 1997, 16 (15) : 4738 - 4745
[43] Parrish S, 2001, RNA, V7, P1397
[44] RNA interference - 2001
Sharp, PA
[J]. GENES & DEVELOPMENT, 2001, 15 (05) : 485 - 490
[45] How cells respond to interferons
Stark, GR
Kerr, IM
Williams, BRG
Silverman, RH
Schreiber, RD
[J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1998, 67 : 227 - 264
[46] RNAi in mouse oocytes and preimplantation embryos: Effectiveness of hairpin dsRNA
Svoboda, P
Stein, P
Schultz, RM
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2001, 287 (05) : 1099 - 1104
[47] RNAi in C-elegans:: Soaking in the genome sequence
Tabara, H
Grishok, A
Mello, CC
[J]. SCIENCE, 1998, 282 (5388) : 430 - 431
[48] Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans
Timmons, L
Court, DL
Fire, A
[J]. GENE, 2001, 263 (1-2) : 103 - 112
[49] Systemic RNAi in C-elegans requires the putative transmembrane protein SID-1
Winston, WM
Molodowitch, C
Hunter, CP
[J]. SCIENCE, 2002, 295 (5564) : 2456 - 2459
[50] RNA as a drug target: methods for biophysical characterization and screening
Xavier, KA
Eder, PS
Giordano, T
[J]. TRENDS IN BIOTECHNOLOGY, 2000, 18 (08) : 349 - 356

← 1 2 3 4 5 6 →