RNAi: gene-silencing in therapeutic intervention

被引：114

作者：

Shuey, DJ ^{[1
]}

McCallus, DE ^{[1
]}

Giordano, T ^{[1
]}

机构：

[1] Nucleon, Malvern, PA 19355 USA

来源：

DRUG DISCOVERY TODAY | 2002年 / 7卷 / 20期

关键词：

D O I：

10.1016/S1359-6446(02)02474-1

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Several rapidly developing RNA interference (RNAi) methodologies hold the promise to selectively inhibit gene expression in mammals. RNAi is an innate cellular process activated when a double-stranded RNA (dsRNA) molecule of greater than 19 duplex nucleotides enters the cell, causing the degradation of not only the invading dsRNA molecule, but also single-stranded (ssRNAs) RNAs of identical sequences, including endogenous mRNAs. As such, RNAi technology is currently being evaluated not only as an extremely powerful instrument for functional genomic analyses, but also as a potentially useful method to develop highly specific dsRNA based gene-silencing therapeutics.

引用

页码：1040 / 1046

页数：7

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