Sample stacking revisited: A personal perspective

被引:123
作者
Chien, RL [1 ]
机构
[1] Caliper Technol Corp, Mountain View, CA 94043 USA
关键词
electrokinetics; electroosmosis; field-amplified sample stacking; microchips; microfluidic device; review; sample preconcentration; sample stacking;
D O I
10.1002/elps.200390057
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
One of the major challenges in capillary electrophoresis and other miniaturization separation techniques is to maintain high detection sensitivity in the increasingly smaller dimension. Numerous on-column sample preconcentrating procedures, based either on electrokinetic focusing or chromatographic effects, have been developed. This review will discuss some practical approaches to sample stacking from a personal perspective. Several recent developments in sample stacking on microfluidic devices are reviewed.
引用
收藏
页码:486 / 497
页数:12
相关论文
共 117 条
[1]   ELECTROOSMOSIS THROUGH PORES WITH NONUNIFORMLY CHARGED WALLS [J].
ANDERSON, JL ;
IDOL, WK .
CHEMICAL ENGINEERING COMMUNICATIONS, 1985, 38 (3-6) :93-106
[2]  
Beckers JL, 2000, ELECTROPHORESIS, V21, P2747, DOI 10.1002/1522-2683(20000801)21:14<2747::AID-ELPS2747>3.0.CO
[3]  
2-Z
[4]  
BOCEK P, 1989, J CHROMATOGR, V470, P309
[5]  
Breadmore MC, 2001, ELECTROPHORESIS, V22, P2464
[6]   Velocity-difference induced focusing of nucleotides in capillary electrophoresis with a dynamic pH junction [J].
Britz-McKibbin, P ;
Bebault, GM ;
Chen, DDY .
ANALYTICAL CHEMISTRY, 2000, 72 (08) :1729-1735
[7]  
Burgi D S, 1996, Methods Mol Biol, V52, P211
[8]   APPLICATION OF SAMPLE STACKING TO GRAVITY INJECTION IN CAPILLARY ELECTROPHORESIS [J].
BURGI, DS ;
CHIEN, RL .
JOURNAL OF MICROCOLUMN SEPARATIONS, 1991, 3 (03) :199-202
[9]   OPTIMIZATION IN SAMPLE STACKING FOR HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS [J].
BURGI, DS ;
CHIEN, RL .
ANALYTICAL CHEMISTRY, 1991, 63 (18) :2042-2047
[10]   Moving chemical reaction boundary and isoelectric focusing - I. Conditional equations for Svensson-Tiselius' differential equation of solute concentration distribution in idealized isoelectric focusing at steady state [J].
Cao, CX .
JOURNAL OF CHROMATOGRAPHY A, 1998, 813 (01) :153-171