Sample stacking revisited: A personal perspective

被引:123
作者
Chien, RL [1 ]
机构
[1] Caliper Technol Corp, Mountain View, CA 94043 USA
关键词
electrokinetics; electroosmosis; field-amplified sample stacking; microchips; microfluidic device; review; sample preconcentration; sample stacking;
D O I
10.1002/elps.200390057
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
One of the major challenges in capillary electrophoresis and other miniaturization separation techniques is to maintain high detection sensitivity in the increasingly smaller dimension. Numerous on-column sample preconcentrating procedures, based either on electrokinetic focusing or chromatographic effects, have been developed. This review will discuss some practical approaches to sample stacking from a personal perspective. Several recent developments in sample stacking on microfluidic devices are reviewed.
引用
收藏
页码:486 / 497
页数:12
相关论文
共 117 条
[11]   FIELD AMPLIFIED SAMPLE INJECTION IN HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS [J].
CHIEN, RL ;
BURGI, DS .
JOURNAL OF CHROMATOGRAPHY, 1991, 559 (1-2) :141-152
[12]   Comment on "Electrokinetic stacking injection of neutral analytes under continuous conductivity conditions" [J].
Chien, RL .
ANALYTICAL CHEMISTRY, 2002, 74 (15) :3929-3930
[13]   ELECTROOSMOTIC PROPERTIES AND PEAK BROADENING IN FIELD-AMPLIFIED CAPILLARY ELECTROPHORESIS [J].
CHIEN, RL ;
HELMER, JC .
ANALYTICAL CHEMISTRY, 1991, 63 (14) :1354-1361
[14]   SAMPLE STACKING OF AN EXTREMELY LARGE INJECTION VOLUME IN HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS [J].
CHIEN, RL ;
BURGI, DS .
ANALYTICAL CHEMISTRY, 1992, 64 (09) :1046-1050
[15]   FIELD-AMPLIFIED POLARITY-SWITCHING SAMPLE INJECTION IN HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS [J].
CHIEN, RL ;
BURGI, DS .
JOURNAL OF CHROMATOGRAPHY, 1991, 559 (1-2) :153-161
[16]   ON-COLUMN SAMPLE CONCENTRATION USING FIELD AMPLIFICATION IN CZE [J].
CHIEN, RL ;
BURGI, DS .
ANALYTICAL CHEMISTRY, 1992, 64 (08) :A489-A496
[17]   Protein analysis with large volume sample stacking with an electrosmotic flow pump: a potential approach for proteomics [J].
Chun, MS ;
Kang, D ;
Kim, Y ;
Chung, D .
MICROCHEMICAL JOURNAL, 2001, 70 (03) :247-253
[18]   Large-volume sample stacking for on-capillary sample enrichment in the determination of naphthalene- and benzenesulfonates in real water samples by capillary zone electrophoresis [J].
Cugat, MJ ;
Borrull, F ;
Calull, M .
ANALYST, 2001, 126 (08) :1312-1317
[20]   MANIPULATION OF SAMPLE FRACTIONS ON A CAPILLARY ELECTROPHORESIS CHIP [J].
EFFENHAUSER, CS ;
MANZ, A ;
WIDMER, HM .
ANALYTICAL CHEMISTRY, 1995, 67 (13) :2284-2287