Synthetic polymer coatings for long-term growth of human embryonic stem cells

被引：276

作者：

Villa-Diaz, Luis G. ^{[2
,3
]}

Nandivada, Himabindu ^{[1
]}

Ding, Jun ^{[2
]}

Nogueira-de-Souza, Naiara C. ^{[2
,4
]}

Krebsbach, Paul H. ^{[3
,5
]}

O'Shea, K. Sue ^{[6
]}

Lahann, Joerg ^{[1
,5
,7
,8
]}

Smith, Gary D. ^{[2
,9
,10
]}

机构：

[1] Univ Michigan, Dept Chem Engn, Ann Arbor, MI 48109 USA

[2] Univ Michigan, Dept Obstet & Ginecol, Ann Arbor, MI 48109 USA

[3] Univ Michigan, Sch Dent, Dept Biol & Mat Sci, Ann Arbor, MI 48109 USA

[4] Barrelos Canc Hosp, Human Biol Res Lab, Sao Paulo, Brazil

[5] Univ Michigan, Dept Biomed Engn, Ann Arbor, MI 48109 USA

[6] Univ Michigan, Dept Cell & Dev Biol, Ann Arbor, MI 48109 USA

[7] Univ Michigan, Dept Mat Sci & Engn, Ann Arbor, MI 48109 USA

[8] Univ Michigan, Dept Macromol Sci & Engn, Ann Arbor, MI 48109 USA

[9] Univ Michigan, Dept Urol, Ann Arbor, MI 48109 USA

[10] Univ Michigan, Reprod Sci Program, Ann Arbor, MI 48109 USA

来源：

NATURE BIOTECHNOLOGY | 2010年 / 28卷 / 06期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

SELF-RENEWAL; BIOMATERIALS; MATRICES; CULTURE;

D O I：

10.1038/nbt.1631

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We report a fully defined synthetic polymer coating, poly[2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium hydroxide] (PMEDSAH), which sustains long-term human embryonic stem (hES) cell growth in several different culture media, including commercially available defined media. The development of a standardized, controllable and sustainable culture matrix for hES cells is an essential step in elucidating mechanisms that control hES cell behavior and in optimizing conditions for biomedical applications of hES cells.

引用

页码：581 / 583

页数：3

共 12 条

[1] Nanoliter-scale synthesis of arrayed biomaterials and application to human embryonic stem cells [J].

Anderson, DG ;

Levenberg, S ;

Langer, R .

NATURE BIOTECHNOLOGY, 2004, 22 (07) :863-866

[2] Recombinant vitronectin is a functionally defined substrate that supports human embryonic stem cell self-renewal via αVβ5 integrin [J].

Braam, Stefan R. ;

Zeinstra, Laura ;

Litjens, Sandy ;

Ward-van Oostwaard, Dorien ;

van den Brink, Stieneke ;

van Laake, Linda ;

Lebrin, Franck ;

Kats, Peter ;

Hochstenbach, Ron ;

Passier, Robert ;

Sonnenberg, Arnoud ;

Mummery, Christine L. .

STEM CELLS, 2008, 26 (09) :2257-2265

[3] Defining Long-Term Maintenance Conditions of Human Embryonic Stem Cells With Arrayed Cellular Microenvironment Technology [J].

Brafman, David A. ;

Shah, Kevan D. ;

Fellner, Thomas ;

Chien, Shu ;

Willert, Karl .

STEM CELLS AND DEVELOPMENT, 2009, 18 (08) :1141-1154

[4] Defined substrates for human embryonic stem cell growth identified from surface arrays [J].

Derda, Ratmir ;

Li, Lingyin ;

Orner, Brendan P. ;

Lewis, Rachel L. ;

Thomson, James A. ;

Kiessling, Laura L. .

ACS CHEMICAL BIOLOGY, 2007, 2 (05) :347-355

[5] Comparison of Biomaterials and Extracellular Matrices as a Culture Platform for Multiple, Independently Derived Human Embryonic Stem Cell Lines [J].

Hakala, Heidi ;

Rajala, Kristiina ;

Ojala, Marisa ;

Panula, Sarita ;

Areva, Sami ;

Kellomaki, Minna ;

Suuronen, Riitta ;

Skottman, Heli .

TISSUE ENGINEERING PART A, 2009, 15 (07) :1775-1785

[6] Hydrogels as artificial matrices for human embryonic stem cell self-renewal [J].

Li, Ying J. ;

Chung, Eugene H. ;

Rodriguez, Ryan T. ;

Firpo, Meri T. ;

Healy, Kevin E. .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2006, 79A (01) :1-5

[7] Derivation of human embryonic stem cells in defined conditions [J].

Ludwig, TE ;

Levenstein, ME ;

Jones, JM ;

Berggren, WT ;

Mitchen, ER ;

Frane, JL ;

Crandall, LJ ;

Daigh, CA ;

Conard, KR ;

Piekarczyk, MS ;

Llanas, RA ;

Thomson, JA .

NATURE BIOTECHNOLOGY, 2006, 24 (02) :185-187

[8] Toward xeno-free culture of human embryonic stem cells [J].

Mallon, Barbara S. ;

Park, Kye-Yoon ;

Chen, Kevin G. ;

Hamilton, Rebecca S. ;

McKay, Ronald D. G. .

INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2006, 38 (07) :1063-1075

[9] Human embryonic stem cells express an immunogenic nonhuman sialic acid [J].

Martin, MJ ;

Muotri, A ;

Gage, F ;

Varki, A .

NATURE MEDICINE, 2005, 11 (02) :228-232

[10] Multiplexed Staining of Live Human Embryonic Stem Cells for Flow Cytometric Analysis of Pluripotency Markers [J].

Prowse, Andrew B. J. ;

Wilson, John ;

Osborne, Geoffrey W. ;

Gray, Peter P. ;

Wolvetang, Ernst J. .

STEM CELLS AND DEVELOPMENT, 2009, 18 (08) :1135-1139

← 1 2 →