Development of a quality evaluation system for Panax quinquefolium. L based on HPLC chromatographic fingerprinting of seven major ginsenosides

A HPLC fingerprinting method based on the distribution and contents of seven major ginsenosides has been developed for the quality evaluation of Panax quinquefolium. L roots cultivated in China, Singapore and Canada. The method is ideally suited for the fingerprinting of P. quinquefolium. L samples or its derived products for sample authentication or quality control, e.g. pharmaceutical stability studies. Different extraction methods have been evaluated and a protocol established to maximize the gensinosides yields in routine operations. Hierarchical clustering analysis of the fingerprints demonstrates that the distribution and contents of ginsenosides in P quinquefolium. L root vary depending on geographic location; and the quality of Jilin-cultivated P quinquefolium. L roots is similar in composition to Canada-cultivated ones, which are generally considered among the best quality in P quinquefolium. L. (c) 2006 Elsevier B.V. All rights reserved.