Reduced phagocytic activity of polymorphonuclear leukocytes in α(1,3) fucosyltransferase VII-deficient mice

被引：3

作者：

Bartunkova, J ^{[1
]}

Maly, P ^{[1
]}

Smetana, K ^{[1
]}

Sedivá, A ^{[1
]}

Klubal, R ^{[1
]}

Mayerová, D ^{[1
]}

Sedlácek, A ^{[1
]}

Splíchalová, V ^{[1
]}

机构：

[1] Charles Univ Prague, Fac Med 2, Inst Immunol, Prague 15006 5, Czech Republic

来源：

APMIS | 2000年 / 108卷 / 06期

关键词：

alpha(1,3)fucosyltransferase VII; selectin ligand; adhesion molecules; gene knock-out; phagocytosis;

D O I：

10.1034/j.1600-0463.2000.d01-76.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Deficiencies in adhesion molecules or their counter-receptors in humans may have severe consequences as exemplified by leukocyte adhesion deficiency (LAD) I or II syndromes. Because such diseases occur with great rarity, animal models are valuable for studying the role of particular adhesion molecules and their natural ligands in immunity. We studied selected immune parameters and general health in mice with a defect in the sialyl-Lewis X antigen (selectin ligand) caused by disruption of the gene encoding alpha(1,3)fucosyltransferase VII (Fuc-TVII). Leukocytes from Fuc-TVII -/- and control mice were tested for adherence to cellophane membranes or polymer particles in vivo and phagocytic activity in vitro. While no difference in adherence was found, the number of neutrophil granulocytes in exudate induced by intraperitoneal injection of polymer beads was reduced in knock-out mice. Moreover, the phagocytic activity in Fuc-TVII -/- mice was significantly reduced. These animals have splenomegaly due to increased hematopoiesis and reduced weight but do not exhibit clinical signs of immunodeficiency. In conclusion, the lack of Fuc-TVII activity leads to several morphological and functional abnormalities without an impact on survival rate.

引用

页码：409 / 416

页数：8

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