Direct injection of solid-phase extraction eluents onto silica columns for the analysis of polar compounds isoniazid and cetirizine in plasma using hydrophilic interaction chromatography with tandem mass spectrometry

被引:45
作者
Li, AC [1 ]
Junga, H [1 ]
Shou, WZ [1 ]
Bryant, MS [1 ]
Jiang, XY [1 ]
Weng, ND [1 ]
机构
[1] Covance Labs Inc, Madison, WI USA
关键词
D O I
10.1002/rcm.1623
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Isoniazid and cetirizine do not retain well on reversed-phase columns due to their high polarity. Silica columns, when operated under hydrophilic interaction conditions, do provide excellent retention of these compounds. We have developed simple and proof of concept analytical methods for the analysis of isoniazid and cetirizine in animal and human plasma, respectively. Both methods employed the approach of direct injection of solid-phase extraction (SPE) organic eluents onto silica columns for analysis, thus eliminating evaporation and reconstitution steps that are typically needed for reversed-phase liquid chromatographic analysis. Isoniazid was extracted from animal plasma samples using a Waters Oasis HLB 96-well plate and then eluted with acetonitrile, while cetirizine was extracted from human plasma with a Waters MCX mu-Elute plate and then eluted with acetonitrile containing 5% concentrated ammonium hydroxide. The direct injection of the SPE eluent onto the analytical column was necessary since significant loss of isoniazid was found during the evaporation and reconstitution steps. The method for isoniazid also enabled ultra-fast analysis due to the relatively low back-pressure exhibited by silica columns even under high flow conditions. Both methods show good linearity, accuracy and precision covering the range of 10-2000ng/mL of isoniazid, and 1-1000ng/mL of cetirizine in plasma. Substantial time savings were realized as a result of both the elimination of the evaporation and reconstitution steps and the fast chromatographic analysis. Copyright (C) 2004 John Wiley Sons, Ltd.
引用
收藏
页码:2343 / 2350
页数:8
相关论文
共 16 条
[1]  
Ackermann Bradley L., 2002, Current Topics in Medicinal Chemistry, V2, P53, DOI 10.2174/1568026023394605
[2]   Comparison of plasma sample purification by manual liquid-liquid extraction, automated 96-well liquid-liquid extraction and automated 96-well solid-phase extraction for analysis by highperformance liquid chromatography with tandem mass spectrometry [J].
Jemal, M ;
Teitz, D ;
Ouyang, Z ;
Khan, S .
JOURNAL OF CHROMATOGRAPHY B, 1999, 732 (02) :501-508
[3]   Liquid chromatographic determination of isoniazid, pyrazinamide and rifampicin from pharmaceutical preparations and blood [J].
Khuhawar, MY ;
Rind, FMA .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2002, 766 (02) :357-363
[4]   Separation of nicotinic acid and six metabolites within 60 seconds using high-flow gradient chromatography on silica column with tandem mass spectrometric detection [J].
Li, AC ;
Chen, YL ;
Junga, H ;
Shou, WZ ;
Jiang, X ;
Naidong, W .
CHROMATOGRAPHIA, 2003, 58 (11-12) :723-731
[5]  
MATZKE GR, 1987, ANN ALLERGY, V59, P25
[6]  
Minikis RM, 2003, LC GC N AM, V21, P1050
[7]   DETERMINATION OF CETIRIZINE IN SERUM USING REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ULTRAVIOLET SPECTROPHOTOMETRIC DETECTION [J].
MONCRIEFF, J .
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1992, 583 (01) :128-130
[8]   Therapeutic isoniazid monitoring using a simple high-performance liquid chromatographic method with ultraviolet detection [J].
Moussa, LA ;
Khassouani, CE ;
Soulaymani, R ;
Jana, M ;
Cassanas, G ;
Alric, R ;
Hüe, B .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2002, 766 (01) :181-187
[9]  
MUSCARA MN, 1995, INT J CLIN PHARM TH, V33, P27
[10]   Novel liquid chromatographic-tandem mass spectrometric methods using silica columns and aqueous-organic mobile phases for quantitative analysis of polar ionic analytes in biological fluids [J].
Naidong, W ;
Shou, W ;
Chen, YL ;
Jiang, XY .
JOURNAL OF CHROMATOGRAPHY B, 2001, 754 (02) :387-399