Identification of the absorbed components and metabolites of Xiao-Ai-Jie-Du decoction and their distribution in rats using ultra high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry

被引：20

作者：

Fan, Huisen ^{[1
,2
]}

Liu, Shijia ^{[3
,4
]}

Shen, Weixing ^{[1
]}

Kang, An ^{[2
]}

Tan, Jiani ^{[1
]}

Li, Liu ^{[1
]}

Liu, Xiao ^{[2
]}

Xu, Changliang ^{[1
]}

Xu, Xuefen ^{[1
]}

Lai, Yueyang ^{[1
]}

Cheng, Haibo ^{[1
]}

Sun, Dongdong ^{[1
]}

机构：

[1] Nanjing Univ Chinese Med, Jiangsu Collaborat Innovat Ctr Tradit Chinese Med, Jiangsu Prov Lab Proved Anticarcinoma Recipe Res, Key Lab Famous Doctors Proved Recipe Evaluat & Tr, Nanjing 210023, Peoples R China

[2] Nanjing Univ Chinese Med, Sch Pharm, Nanjing 210023, Peoples R China

[3] Nanjing Univ Chinese Med, Affiliated Hosp, Nanjing 210029, Peoples R China

[4] China Pharmaceut Univ, State Key Lab Nat Med, Nanjing 210023, Peoples R China

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2020年 / 179卷

基金：

中国国家自然科学基金;

关键词：

Absorbed components; Metabolites; Identification; UHPLC-Q-TOF-MS; Xiao-Ai-Jie-Du formula; CHEMICAL-CONSTITUENTS; RAPID CHARACTERIZATION; MS METHOD; HPLC-DAD; PLASMA; MEDICINE; STRATEGY; URINE; PHENANTHRENES; TUBERS;

D O I：

10.1016/j.jpba.2019.112984

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学];

摘要：

Xiao-Ai-Jie-Du decoction (XAJDD), a traditional Chinese medicine formula, has long been used for the treatment of hepatocarcinoma, gastric cancer and colorectal cancer. It is composed of six herbal medicines, including Scutellariae Barbatae Herba, Pseudostellariae Radix, Ophiopogonis Radix, Cremastrae Pseudobulbus, Curcumae Rhizoma and Akebiae Fructus. Despite the in-depth study on its pharmacological effects on cancer prevention and treatment, the comprehensive analysis of the chemical components and the absorbed bioactive constituents are not well studied. Thus, an ultra-high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) method was established to detect and identify the chemical constituents in XAJDD. The absorbed components and metabolites after oral administration of XAJDD in rats were also studied. In total, 102 components were identified or tentatively characterized in XAJDD, including 30 flavonoids, 19 triterpenoids, 12 organic acids, 9 steroidal saponins, 9 cyclic peptides, 7 phenanthrenes, 5 amino acids, 3 alkaloids and 8 other compounds. After analysing the metabolites in rat plasma and urine after oral administration of XAJDD, a total of 70 compounds were identified, including 15 primary components and 55 metabolites, and metabolic pathways, including hydrogenation, hydroxylation, methylation, sulfonation, and glucuronidation were evaluated. Among these, methylation and glucuronidation were the main metabolic pathways. In conclusion, the developed UHPLC-Q-TOF-MS method with high sensitivity and resolution is suitable for identifying and characterizing the chemical constituents of XAJDD in vitro and characterizing the primary components and their metabolites in vivo; moreover, the results will provide essential data for further studying the relationship between the chemical components and pharmacological activity of XAJDD. (C) 2019 Elsevier B.V. All rights reserved.

引用

页数：14

共 36 条

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Absorption, conjugation and excretion of the flavanones, naringenin and hesperetin from α-rhamnosidase-treated orange juice in human subjects [J].