Intracellular Bioconjugation of Targeted Proteins with Semiconductor Quantum Dots

被引:86
作者
Boeneman, Kelly [1 ]
Delehanty, James B. [1 ]
Susumu, Kimihiro [2 ]
Stewart, Michael H. [2 ]
Medintz, Igor L. [1 ]
机构
[1] USN, Res Lab, Ctr Biomol Sci & Engn, Washington, DC 20375 USA
[2] USN, Res Lab, Div Opt Sci, Washington, DC 20375 USA
关键词
RESONANCE ENERGY-TRANSFER; FLUORESCENT PROTEIN; TAGGED PROTEINS; LIVE CELLS; STABILITY; PROGRESS;
D O I
10.1021/ja100201w
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
We demonstrate controlled in vivo bioconjugation of a targeted intracellular protein to semiconductor quantum dots (QDs). Metal-affinity driven coordination of oligohistidine-appended proteins for chelated divalent cations was exploited to facilitate this interaction. Monomeric mCherry red fluorescent protein recombinantly engineered to express an N-terminal hexahistidine sequence was expressed from a eukaryotic plasmid vector following transfection into COS-1 cells. QDs solubilized with a carboxylated polymeric ligand and pretreated with Ni2+ were then microinjected into the mCherry-expressing COS-1 cells. Forster resonance energy transfer (FRET) between the central QD donors and mCherry acceptors specifically coordinated to their surface was utilized to probe and confirm intracellular conjugate formation. We unexpectedly found that mCherry attachment to the QDs also substantially improves its resistance to photobleaching. This proof-of-concept, highlighting targeted intracellular bioconjugation to QDs, suggests that many cytoplasmic proteins expressing the ubiquitous hexahistidine affinity handle can be specifically attached to QDs in vivo. This approach can facilitate Long-term monitoring of their spacio-temporal activity or, alternatively, allow engineering and in situ assembly of designer chimeric QD-fluorescent protein sensors.
引用
收藏
页码:5975 / +
页数:5
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