High-resolution DNA melt curve analysis of the clustered, regularly interspaced short-palindromic-repeat locus of Campylobacter jejuni

被引：89

作者：

Price, Erin P.

Smith, Helen

Huygens, Flavia

Giffard, Philip M.

机构：

[1] Queensland Univ Technol, Cooperat Res Ctr Diagnost, Inst Hlth & Biomed Innovat, Brisbane, Qld, Australia

[2] Queensland Hlth Sci Serv, Coopers Plains, Qld, Australia

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2007年 / 73卷 / 10期

关键词：

D O I：

10.1128/AEM.02702-06

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A novel method for genotyping the clustered, regularly interspaced short-palindromic-repeat (CRISPR) locus of Campylobacter jejuni is described. Following real-time PCR, CRISPR products were subjected to high-resolution melt (HRM) analysis, a new technology that allows precise melt profile determination of amplicons. This investigation shows that the CRISPR HRM assay provides a powerful addition to existing C. jejuni genotyping methods and emphasizes the potential of HRM for genotyping short sequence repeats in other species.

引用

页码：3431 / 3436

页数：6

共 20 条

[1] Major structural differences and novel potential virulence mechanisms from the genomes of multiple Campylobacter species [J].

Fouts, DE ;

Mongodin, EF ;

Mandrell, RE ;

Miller, WG ;

Rasko, DA ;

Ravel, J ;

Brinkac, LM ;

DeBoy, RT ;

Parker, CT ;

Daugherty, SC ;

Dodson, RJ ;

Durkin, AS ;

Madupu, R ;

Sullivan, SA ;

Shetty, JU ;

Ayodeji, MA ;

Shvartsbeyn, A ;

Schatz, MC ;

Badger, JH ;

Fraser, CM ;

Nelson, KE .

PLOS BIOLOGY, 2005, 3 (01) :72-85

[2] Legionella confirmation using real-time PCR and SYTO9 is an alternative to current methodology [J].

Giglio, S ;

Monis, PT ;

Saint, CP .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2005, 71 (12) :8944-8948

[3] Demonstration of preferential binding of SYBR Green I to specific DNA fragments in real-time multiplex PCR [J].

Giglio, S ;

Monis, PT ;

Saint, CP .

NUCLEIC ACIDS RESEARCH, 2003, 31 (22) :e136

[4] Amplicon DNA melting analysis for mutation scanning and genotyping: Cross-platform comparison of instruments and dyes [J].

Herrmann, MG ;

Durtschi, JD ;

Bromley, LK ;

Wittwer, CT ;

Voelkerding, KV .

CLINICAL CHEMISTRY, 2006, 52 (03) :494-503

[5] mecA locus diversity in methicillin-resistant Staphylococcus aureus isolates in Brisbane, Australia, and the development of a novel diagnostic procedure for the Western Samoan phage pattern clone [J].

Huygens, F ;

Stephens, AJ ;

Nimmo, GR ;

Giffard, PM .

JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (05) :1947-1955

[6] Staphylococcus aureus genotyping using novel real-time PCR formats [J].

Huygens, Flavia ;

Inman-Bamber, John ;

Nimmo, Graeme R. ;

Munckhof, Wendy ;

Schooneveldt, Jacqueline ;

Harrison, Bruce ;

McMahon, Jennifer A. ;

Giffard, Philip M. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (10) :3712-3719

[7] Identification of genes that are associated with DNA repeats in prokaryotes [J].

Jansen, R ;

van Embden, JDA ;

Gaastra, W ;

Schouls, LM .

MOLECULAR MICROBIOLOGY, 2002, 43 (06) :1565-1575

[8] mlstdbNet - distributed multi-locus sequence typing (MLST) databases [J].

Jolley, KA ;

Chan, MS ;

Maiden, MCJ .

BMC BIOINFORMATICS, 2004, 5 (1)

[9] High resolution melting analysis for the rapid and sensitive detection of mutations in clinical samples:: KRAS codon 12 and 13 mutations in non-small cell lung cancer [J].

Krypuy, Michael ;

Newnham, Genni M. ;

Thomas, David M. ;

Conron, Matthew ;

Dobrovic, Alexander .

BMC CANCER, 2006, 6 (1)

[10] Intervening sequences of regularly spaced prokaryotic repeats derive from foreign genetic elements [J].

Mojica, FJM ;

Díez-Villaseñor, C ;

García-Martínez, J ;

Soria, E .

JOURNAL OF MOLECULAR EVOLUTION, 2005, 60 (02) :174-182

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