The coiled-coil membrane protein golgin-84 is a novel rab effector required for Golgi ribbon formation

被引:187
作者
Diao, A
Rahman, D
Pappin, DJC
Lucocq, J
Lowe, M
机构
[1] Univ Manchester, Sch Biol Sci, Manchester M13 9PT, Lancs, England
[2] Univ Dundee, Sch Life Sci, Dundee DD1 5EH, Scotland
[3] Univ London Imperial Coll Sci Technol & Med, Hammersmith Hosp, MRC, Clin Sci Ctr,Proteom Sect, London W12 0NN, England
关键词
Golgi apparatus; golgin; mitosis; Golgi structure; protein phosphorylation;
D O I
10.1083/jcb.200207045
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Fragmentation of the mammalian Golgi apparatus during mitosis requires the phosphorylation of a specific subset of Golgi-associated proteins. We have used a biochemical approach to characterize these proteins and report here the identification of golgin-84 as a novel mitotic target. Using cryoelectron microscopy we could localize golgin-84 to the cis-Golgi network and found that it is enriched on tubules emanating from the lateral edges of, and often connecting, Golgi stacks. Golgin-84 binds to active rab1 but not cis-Golgi matrix proteins. Overexpression or depletion of golgin-84 results in fragmentation of the Golgi ribbon. Strikingly, the Golgi ribbon is converted into mini-stacks constituting only similar to25% of the volume of a normal Golgi apparatus upon golgin-84 depletion. These mini-stacks are able to carry out protein transport, though with reduced efficiency compared with a normal Golgi apparatus. Our results suggest that golgin-84 plays a key role in the assembly and maintenance of the Golgi ribbon in mammalian cells.
引用
收藏
页码:201 / 212
页数:12
相关论文
共 56 条