Imaging proteins in vivo using fluorescence lifetime microscopy

被引：101

作者：

Festy, Frederic

Ameer-Beg, Simon M.

Ng, Tony

Suhling, Klaus ^{[1
]}

机构：

[1] Kings Coll London, Randall Div Cell & Mol Biophys, London SE1 1UL, England

[2] Kings Coll London, Richard Dimbleby Dept Canc Res, Div Canc Studies, London SE1 1UL, England

[3] Kings Coll London, Dept Phys, London WC2R 2LS, England

[4] Kings Coll London, Inst Dent, Biomat & Biomimet Dept, London SE1 9RT, England

来源：

MOLECULAR BIOSYSTEMS | 2007年 / 3卷 / 06期

基金：

英国医学研究理事会; 英国工程与自然科学研究理事会;

关键词：

D O I：

10.1039/b617204k

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fluorescence lifetime imaging (FLIM) represents a key optical technique for imaging proteins and protein interaction in vivo. We review the principles and recent advances in the application of the technique, instrumentation and molecular probe development.

引用

收藏

页码：381 / 391

页数：11

相关论文

共 118 条

[1] How the Confocal Laser Scanning Microscope entered Biological Research [J].

Amos, WB ;

White, JG .

BIOLOGY OF THE CELL, 2003, 95 (06) :335-342

[2] Fluorescence lifetime imaging microscopy: spatial resolution of biochemical processes in the cell [J].

Bastiaens, PIH ;

Squire, A .

TRENDS IN CELL BIOLOGY, 1999, 9 (02) :48-52

[3] Observing proteins in their natural habitat: the living cell [J].

Bastiaens, PIH ;

Pepperkok, R .

TRENDS IN BIOCHEMICAL SCIENCES, 2000, 25 (12) :631-637

[4]

Becker W., 2005, SPRINGER SERIES CHEM, V81, DOI 10.1007/3-540-28882-1

[5] Quantitative 3D mapping of fluidic temperatures within microchannel networks using fluorescence lifetime imaging [J].

Benninger, RKP ;

Koç, Y ;

Hofmann, O ;

Requejo-Isidro, J ;

Neil, MAA ;

French, PMW ;

deMello, AJ .

ANALYTICAL CHEMISTRY, 2006, 78 (07) :2272-2278

[6] Time-resolved fluorescence imaging of solvent interactions in microfluidic devices [J].

Benninger, RKP ;

Hofmann, O ;

McGinty, J ;

Requejo-Isidro, J ;

Munro, I ;

Neil, MAA ;

deMello, AJ ;

French, PMW .

OPTICS EXPRESS, 2005, 13 (16) :6275-6285

[7] Fluorescence imaging of two-photon linear dichroism:: Cholesterol depletion disrupts molecular orientation in cell membranes [J].

Benninger, RKP ;

Önfelt, B ;

Neil, MAA ;

Davis, DM ;

French, PMW .

BIOPHYSICAL JOURNAL, 2005, 88 (01) :609-622

[8]

BENNINGER RKP, 2007, ANGEW CHEM INT ED, V46

[9] Imaging intracellular fluorescent proteins at nanometer resolution [J].

Betzig, Eric ;

Patterson, George H. ;

Sougrat, Rachid ;

Lindwasser, O. Wolf ;

Olenych, Scott ;

Bonifacino, Juan S. ;

Davidson, Michael W. ;

Lippincott-Schwartz, Jennifer ;

Hess, Harald F. .

SCIENCE, 2006, 313 (5793) :1642-1645

[10] Simultaneous two-photon spectral and lifetime fluorescence microscopy [J].

Bird, DK ;

Eliceiri, KW ;

Fan, CH ;

White, JG .

APPLIED OPTICS, 2004, 43 (27) :5173-5182

← 1 2 3 4 5 6 7 8 9 10 →