Quantitative PCR analysis of different splice forms of NFAT5 revealed specific gene expression in fetal and adult brain

被引:13
作者
Dalski, A
Wagner, HJ
Schwinger, E
Zühlke, C
机构
[1] Med Univ Lubeck, Inst Humangenet, D-23538 Lubeck, Germany
[2] Med Univ Lubeck, Klin Kinderheilkunde, D-23538 Lubeck, Germany
来源
MOLECULAR BRAIN RESEARCH | 2000年 / 83卷 / 1-2期
关键词
NFAT5; quantitative PCR analysis; differential splicing; SCA4;
D O I
10.1016/S0169-328X(00)00202-3
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
To investigate sequences or mutations associated with neurodegenerative disorders, we performed analyses for the NFAT5 gene, which is located in the candidate region for the autosomal dominantly inherited spinocerebellar ataxia type 4 (SCA4). PCR based expression analyses detected NFAT5 transcripts with alternative splicing in 27 fetal and adult human tissues. Interestingly, by using quantitative methods on cDNA from fetal and adult human brain a significant difference at the expression level fur one splice form could be shown. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:125 / 127
页数:3
相关论文
共 11 条
[1]  
Flanigan K, 1996, AM J HUM GENET, V59, P392
[2]  
HEBINCK A, IN PRESS CYTOGENET C
[3]   ANDROGEN RECEPTOR GENE-MUTATIONS IN X-LINKED SPINAL AND BULBAR MUSCULAR-ATROPHY [J].
LASPADA, AR ;
WILSON, EM ;
LUBAHN, DB ;
HARDING, AE ;
FISCHBECK, KH .
NATURE, 1991, 352 (6330) :77-79
[4]  
LIE YS, 1998, ADV QUANTITATIVE PCR, P43
[5]   NFAT5, a constitutively nuclear NFAT protein that does not cooperate with Fos and Jun [J].
López-Rodríguez, C ;
Aramburu, J ;
Rakeman, AS ;
Rao, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (13) :7214-7219
[6]   Tonicity-responsive enhancer binding protein, a Rel-like protein that stimulates transcription in response to hypertonicity [J].
Miyakawa, H ;
Woo, SK ;
Dahl, SC ;
Handler, JS ;
Kwon, HM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (05) :2538-2542
[7]   Prediction of the codling sequences of unidentified human genes.: XVI.: The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro [J].
Nagase, T ;
Kikuno, R ;
Ishikawa, KI ;
Hirosawa, M ;
Ohara, O .
DNA RESEARCH, 2000, 7 (01) :65-73
[8]   The SCA8 transcript is an antisense RNA to a brain-specific transcript encoding a novel actin-binding protein (KLHL1) [J].
Nemes, JP ;
Benzow, KA ;
Koob, MD .
HUMAN MOLECULAR GENETICS, 2000, 9 (10) :1543-1551
[9]   Sensory ataxia and muscle spindle agenesis in mice lacking the transcription factor Egr3 [J].
Tourtellotte, WG ;
Milbrandt, J .
NATURE GENETICS, 1998, 20 (01) :87-91
[10]   Cloning of novel splice variants of mouse mGluR1 [J].
Zhu, H ;
Ryan, K ;
Chen, S .
MOLECULAR BRAIN RESEARCH, 1999, 73 (1-2) :93-103