Correlation of mRNA and protein in complex biological samples

被引：1382

作者：

Maier, Tobias ^{[1
]}

Guell, Marc ^{[1
]}

Serrano, Luis ^{[1
]}

机构：

[1] Ctr Genom Regulat, Syst Biol Unit, Barcelona 08003, Spain

来源：

FEBS LETTERS | 2009年 / 583卷 / 24期

基金：

欧洲研究理事会;

关键词：

mRNA; Protein; Quantitative; Systems biology; Genomics; Proteomics; TANDEM MASS-SPECTROMETRY; YEAST SACCHAROMYCES-CEREVISIAE; CODON ADAPTATION INDEX; GENOME-WIDE ANALYSIS; N-END RULE; GENE-EXPRESSION; QUANTITATIVE PROTEOMICS; ABSOLUTE QUANTIFICATION; DESULFOVIBRIO-VULGARIS; RELATIVE QUANTITATION;

D O I：

10.1016/j.febslet.2009.10.036

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The correlation between mRNA and protein abundances in the cell has been reported to be notoriously poor. Recent technological advances in the quantitative analysis of mRNA and protein species in complex samples allow the detailed analysis of this pathway at the center of biological systems. We give an overview of available methods for the identification and quantification of free and ribosome-bound mRNA, protein abundances and individual protein turnover rates. We review available literature on the correlation of mRNA and protein abundances and discuss biological and technical parameters influencing the correlation of these central biological molecules. (c) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：3966 / 3973

页数：8

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