Simple high-performance liquid chromatographic column-switching technique for the online immunoaffinity extraction and analysis of flunitrazepam and its main metabolites in urine

被引:23
作者
Deinl, I
Angermaier, L
Franzelius, C
Machbert, G
机构
[1] Inst Forens Med, D-91054 Erlangen, Germany
[2] Boehringer Mannheim Diagnost, D-82327 Tutzing, Germany
来源
JOURNAL OF CHROMATOGRAPHY B | 1997年 / 704卷 / 1-2期
关键词
flunitrazepam; benzodiazepines;
D O I
10.1016/S0378-4347(97)00429-5
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A sensitive, simple and rapid method without sample pretreatment is presented for the simultaneous determination of flunitrazepam and its main metabolites (norflunitrazepam, 7-amino- and 7-acetamidoflunitrazepam) in urine. The single-step procedure is based on a column-switching technique which uses an immobilized antibody in an extraction column following concentration on a precolumn and separation on an analytical column. UV detection was performed at 254 nm. The reusability of the antibody exceeds 88 runs and a complete analysis was performed in less than 40 min. The method shows coefficients of variation below 9.9% and rates of recovery greater than 92% tested at the level of 50 ng/ml urine. The limit of detection was below 2 ng/ml urine for the four compounds. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:251 / 258
页数:8
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