Supercritical carbon dioxide extraction of colchicine and related alkaloids from seeds of Colchicum autumnale L.

被引:37
作者
Ellington, E [1 ]
Bastida, J [1 ]
Viladomat, F [1 ]
Codina, C [1 ]
机构
[1] Univ Barcelona, Fac Pharm, Dept Nat Prod Plant Biol & Epaphol, E-08028 Barcelona, Spain
关键词
supercritical carbon dioxide extraction; colchicine; 3-demethylcolchicine; colchicoside; HPLC analysis; Colchicum autumnale;
D O I
10.1002/pca.702
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A method for the extraction of the alkaloids colchicine, 3-demethylcolchicine and colchicoside from seeds of Colchicum autumnale by supercritical carbon dioxide has been established. Several parameters such as pressure, temperature, percentage of modifier and extraction time have been examined. Two extraction steps with constant carbon dioxide density (0.90 g/mL) and flux (1.5 mL/min) were required to extract the alkaloids in 110 min using 31% methanol as modifier. The quantitative determination of the alkaloids was performed by HPLC; the percentages of recovery were higher than 98% for the three alkaloids. This extraction procedure was compared with a conventional method involving maceration and sonication, and the same levels of alkaloids were obtained in each case. The supercritical carbon dioxide method is, however, very efficient, more rapid and more environmentally friendly than conventional methods. Copyright (C) 2003 John Wiley Sons, Ltd.
引用
收藏
页码:164 / 169
页数:6
相关论文
共 32 条
[21]   The interaction between lipid derivatives of colchicine and tubulin: Consequences of the interaction of the alkaloid with lipid membranes [J].
Mons, S ;
Veretout, F ;
Carlier, MF ;
Erk, I ;
Lepault, J ;
Trudel, E ;
Salesse, C ;
Ducray, P ;
Mioskowski, C ;
Lebeau, L .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2000, 1468 (1-2) :381-395
[22]  
MOORE S, 1994, CHEM ENG-NEW YORK, V101, P32
[23]   Matrix and modifier effects in the supercritical fluid extraction of cocaine and benzoylecgonine from human hair [J].
Morrison, JF ;
Chesler, SN ;
Yoo, WJ ;
Selavka, CM .
ANALYTICAL CHEMISTRY, 1998, 70 (01) :163-172
[24]  
Mukhopadhyay M., 2000, NATURAL EXTRACTS USI
[25]   SOME APPLICATIONS OF SUPERCRITICAL FLUID EXTRACTION [J].
NDIOMU, DP ;
SIMPSON, CF .
ANALYTICA CHIMICA ACTA, 1988, 213 (1-2) :237-243
[26]   Supercritical fluid extraction and fractionation of essential oils and related products [J].
Reverchon, E .
JOURNAL OF SUPERCRITICAL FLUIDS, 1997, 10 (01) :1-37
[27]   Extraction of purine alkaloids from mate (Ilex paraguariensis) using supercritical CO2 [J].
Saldaña, MDA ;
Mohamed, RS ;
Baer, MG ;
Mazzafera, P .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1999, 47 (09) :3804-3808
[28]  
SARIN YK, 1974, CURR SCI INDIA, V43, P87
[29]   ISOLATION OF VINDOLINE FROM CATHARANTHUS-ROSEUS BY SUPERCRITICAL FLUID EXTRACTION [J].
SONG, KM ;
PARK, SW ;
HONG, WH ;
LEE, H ;
KWAK, SS ;
LIU, JR .
BIOTECHNOLOGY PROGRESS, 1992, 8 (06) :583-586
[30]   SUBSTANCES FROM PLANTS OF SUBFAMILY WURMBAEOIDEAE AND THEIR DERIVATIVES .79. ALKALOIDS OF PLANT GLORIOSA-SUPERBA L [J].
THAKUR, RS ;
POTESILOVA, H ;
SANTAVY, F .
PLANTA MEDICA, 1975, 28 (03) :201-208