Characterization of novel neopullulanase from Bacillus polymyxa

被引：13

作者：

Yebra, MJ ^{[1
]}

Arroyo, J ^{[1
]}

Sanz, P ^{[1
]}

Prieto, JA ^{[1
]}

机构：

[1] CSIC,INST AGROQUIM & TECNOL ALIMENTOS,BURJASSOT 46100,SPAIN

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1997年 / 68卷 / 1-2期

关键词：

extracellular enzyme; neopullulanase; panose; pullulan; Bacillus polymyxa;

D O I：

10.1007/BF02785984

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bacillus polymyxa CECT 155 produces an extracellular neopullulanase activity that degrades pullulan to panose. This activity was stimulated by the presence of pullulan in the culture, and repressed by glucose. The apparent mot wt determined for the enzyme was 58 kDa. The optimum pH and temperature for neopullulanase activity were pH 6.0 and 50 degrees C, respectively. The enzyme was stable in a pH range of 4.0-8.0, and temperatures up to 60 degrees C. These properties make it suitable for the saccharification processes in the starch industries.

引用

页码：113 / 120

页数：8

共 21 条

[1] AMYLASES, ALPHA AND BETA [J].

BERNFELD, P .

METHODS IN ENZYMOLOGY, 1955, 1 :149-158

[2] ACID PULLULANASE FROM BACILLUS-POLYMYXA MIR-23 [J].

CASTRO, GR ;

SANTOPIETRO, LMD ;

SINERIZ, F .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1992, 37 (03) :227-233

[3]

Fogarty W.M., 1980, ECON MICROBIOL, V5, P115

[4]

FOGARTY WM, 1990, MICROBIAL ENZYMES BI, P72

[5]

HEBEDA RE, 1991, CEREAL FOOD WORLD, V36, P619

[6]

HEBEDA RE, 1990, CEREAL FOOD WORLD, V35, P453

[7] PATTERN OF ACTION OF BACILLUS-STEAROTHERMOPHILUS NEOPULLULANASE ON PULLULAN [J].

IMANAKA, T ;

KURIKI, T .

JOURNAL OF BACTERIOLOGY, 1989, 171 (01) :369-374

[8] ENZYMIC STARCH UTILIZATION AND GENETIC-ENGINEERING [J].

KENNEDY, JF ;

CABALDA, VM ;

WHITE, CA .

TRENDS IN BIOTECHNOLOGY, 1988, 6 (08) :184-189

[9] CULTURAL METHOD FOR THE DETECTION OF PULLULAN - DEGRADING ENZYMES IN BACTERIA AND ITS APPLICATION TO THE GENUS BACILLUS [J].

MORGAN, FJ ;

ADAMS, KR ;

PRIEST, FG .

JOURNAL OF APPLIED BACTERIOLOGY, 1979, 46 (02) :291-294

[10] ENZYME AND MICROBIAL SYSTEMS INVOLVED IN STARCH PROCESSING [J].

NIGAM, P ;

SINGH, D .

ENZYME AND MICROBIAL TECHNOLOGY, 1995, 17 (09) :770-778

← 1 2 3 →