The puromycin route to assess stereo- and regiochemical constraints on peptide bond formation in eukaryotic ribosomes

被引：48

作者：

Starck, SR ^{[1
]}

Qi, X ^{[1
]}

Olsen, BN ^{[1
]}

Roberts, RW ^{[1
]}

机构：

[1] CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2003年 / 125卷 / 27期

关键词：

D O I：

10.1021/ja034817e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We synthesized a series of puromycin analogues to probe the chemical specificity of the ribosome in an intact eukaryotic translation system. These studies reveal that both d-enantiomers and β-amino acid analogues can be incorporated into protein, and provide a quantitative means to rank natural and unnatural residues. Modeling of a d-amino acid analogue into the 50S ribosomal subunit indicates that steric clash may provide part of the chiral discrimination. The data presented provide one metric of the chiral and regiospecificity of mammalian ribosomes. Copyright © 2003 American Chemical Society.

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页码：8090 / 8091

页数：2

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