The puromycin route to assess stereo- and regiochemical constraints on peptide bond formation in eukaryotic ribosomes

被引：48

作者：

Starck, SR ^{[1
]}

Qi, X ^{[1
]}

Olsen, BN ^{[1
]}

Roberts, RW ^{[1
]}

机构：

[1] CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2003年 / 125卷 / 27期

关键词：

D O I：

10.1021/ja034817e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We synthesized a series of puromycin analogues to probe the chemical specificity of the ribosome in an intact eukaryotic translation system. These studies reveal that both d-enantiomers and β-amino acid analogues can be incorporated into protein, and provide a quantitative means to rank natural and unnatural residues. Modeling of a d-amino acid analogue into the 50S ribosomal subunit indicates that steric clash may provide part of the chiral discrimination. The data presented provide one metric of the chiral and regiospecificity of mammalian ribosomes. Copyright © 2003 American Chemical Society.

引用

收藏

页码：8090 / 8091

页数：2

相关论文

共 34 条

[21] In vitro selection of mRNA display libraries containing an unnatural amino acid [J].

Li, SW ;

Millward, S ;

Roberts, R .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (34) :9972-9973

[22] PROTEIN-BIOSYNTHESIS WITH CONFORMATIONALLY RESTRICTED AMINO-ACIDS [J].

MENDEL, D ;

ELLMAN, J ;

SCHULTZ, PG .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1993, 115 (10) :4359-4360

[23] PUROMYCIN INHIBITION OF PROTEIN SYNTHESIS - INCORPORATION OF PUROMYCIN INTO PEPTIDE CHAINS [J].

NATHANS, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1964, 51 (04) :585-&

[24] STRUCTURAL REQUIREMENTS FOR PUROMYCIN INHIBITION OF PROTEIN SYNTHESIS [J].

NATHANS, D ;

NEIDLE, A .

NATURE, 1963, 197 (487) :1076-&

[25] The structural basis of ribosome activity in peptide bond synthesis [J].

Nissen, P ;

Hansen, J ;

Ban, N ;

Moore, PB ;

Steitz, TA .

SCIENCE, 2000, 289 (5481) :920-930

[26] A GENERAL-METHOD FOR SITE-SPECIFIC INCORPORATION OF UNNATURAL AMINO-ACIDS INTO PROTEINS [J].

NOREN, CJ ;

ANTHONYCAHILL, SJ ;

GRIFFITH, MC ;

SCHULTZ, PG .

SCIENCE, 1989, 244 (4901) :182-188

[27] NICOTINIC RECEPTOR-BINDING SITE PROBED WITH UNNATURAL AMINO-ACID-INCORPORATION IN INTACT-CELLS [J].

NOWAK, MW ;

KEARNEY, PC ;

SAMPSON, JR ;

SAKS, ME ;

LABARCA, CG ;

SILVERMAN, SK ;

ZHONG, W ;

THORSON, JS ;

ABELSON, JN ;

DAVIDSON, N ;

SCHULTZ, PG ;

DOUGHERTY, DA ;

LESTER, HA .

SCIENCE, 1995, 268 (5209) :439-442

[28] Ribosomal peptidyl transferase can withstand mutations at the putative catalytic nucleotide [J].

Polacek, N ;

Gaynor, M ;

Yassin, A ;

Mankin, AS .

NATURE, 2001, 411 (6836) :498-501

[29] PREPARATION OF MISACYLATED AMINOACYL-TRANSFER RNA PHES USEFUL AS PROBES OF THE RIBOSOMAL ACCEPTOR SITE [J].

ROESSER, JR ;

XU, C ;

PAYNE, RC ;

SURRATT, CK ;

HECHT, SM .

BIOCHEMISTRY, 1989, 28 (12) :5185-5195

[30] Puromycin oligonucleotides reveal steric restrictions for ribosome entry and multiple modes of translation inhibition [J].

Starck, SR ;

Roberts, RW .

RNA, 2002, 8 (07) :890-903

← 1 2 3 4 →