Realizing the Promise of RNAi High Throughput Screening

被引:8
作者
Bakal, Chris [1 ]
Perrimon, Norbert [2 ]
机构
[1] Inst Canc Res, Dynam Cell Syst Team, Sect Cell & Mol Biol, London SW3 6JB, England
[2] Harvard Univ, Sch Med, Howard Hughes Med Inst, Dept Genet, Boston, MA 02115 USA
关键词
ENDOCYTOSIS;
D O I
10.1016/j.devcel.2010.04.005
中图分类号
Q2 [细胞生物学];
学科分类号
071013 [干细胞生物学];
摘要
Recently reporting in Nature, Collinet et al. describes the application of quantitative multiparametric methods to a genome-wide RNAi screen for regulators of endocytosis. The study illustrates the power of this approach beyond the identification of new endocytic components to providing insights into the design principles of the endocytic system.
引用
收藏
页码:506 / 507
页数:2
相关论文
共 9 条
[1]
Phosphorylation networks regulating JNK activity in diverse genetic backgrounds [J].
Bakal, Chris ;
Linding, Rune ;
Llense, Flora ;
Heffern, Elleard ;
Martin-Blanco, Enrique ;
Pawson, Tony ;
Perrimon, Norbert .
SCIENCE, 2008, 322 (5900) :453-456
[2]
Quantitative morphological signatures define local signaling networks regulating cell morphology [J].
Bakal, Chris ;
Aach, John ;
Church, George ;
Perrimon, Norbert .
SCIENCE, 2007, 316 (5832) :1753-1756
[3]
Systems survey of endocytosis by multiparametric image analysis [J].
Collinet, Claudio ;
Stoeter, Martin ;
Bradshaw, Charles R. ;
Samusik, Nikolay ;
Rink, Jochen C. ;
Kenski, Denise ;
Habermann, Bianca ;
Buchholz, Frank ;
Henschel, Robert ;
Mueller, Matthias S. ;
Nagel, Wolfgang E. ;
Fava, Eugenio ;
Kalaidzidis, Yannis ;
Zerial, Marino .
NATURE, 2010, 464 (7286) :243-U123
[4]
Predictive models of molecular machines involved in Caenorhabditis elegans early embryogenesis [J].
Gunsalus, KC ;
Ge, H ;
Schetter, AJ ;
Goldberg, DS ;
Han, JDJ ;
Hao, T ;
Berriz, GF ;
Bertin, N ;
Huang, J ;
Chuang, LS ;
Li, N ;
Mani, R ;
Hyman, AA ;
Sönnichsen, B ;
Echeverri, CJ ;
Roth, FP ;
Vidal, M ;
Piano, F .
NATURE, 2005, 436 (7052) :861-865
[5]
Drosophila genome-wide RNAi screens:: Are they delivering the promise? [J].
Mathey-Prevot, B. ;
Perrimon, N. .
COLD SPRING HARBOR SYMPOSIA ON QUANTITATIVE BIOLOGY, 2006, 71 :141-148
[6]
Gene clustering based on RNAi phenotypes of ovary-enriched genes in C-elegans [J].
Piano, F ;
Schetter, AJ ;
Morton, DG ;
Gunsalus, KC ;
Reinke, V ;
Kim, SK ;
Kemphues, KJ .
CURRENT BIOLOGY, 2002, 12 (22) :1959-1964
[7]
The endocytic matrix [J].
Scita, Giorgio ;
Di Fiore, Pier Paolo .
NATURE, 2010, 463 (7280) :464-473
[8]
Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans [J].
Sönnichsen, B ;
Koski, LB ;
Walsh, A ;
Marschall, P ;
Neumann, B ;
Brehm, M ;
Alleaume, AM ;
Artelt, J ;
Bettencourt, P ;
Cassin, E ;
Hewitson, M ;
Holz, C ;
Khan, M ;
Lazik, S ;
Martin, C ;
Nitzsche, B ;
Ruer, M ;
Stamford, J ;
Winzi, M ;
Heinkel, R ;
Röder, M ;
Finell, J ;
Häntsch, H ;
Jones, SJM ;
Jones, M ;
Piano, F ;
Gunsalus, KC ;
Oegema, K ;
Gönczy, P ;
Coulson, A ;
Hyman, AA ;
Echeverri, CJ .
NATURE, 2005, 434 (7032) :462-469
[9]
Endocytosis and signalling: intertwining molecular networks [J].
Sorkin, Alexander ;
von Zastrow, Mark .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2009, 10 (09) :609-622