A platform for accurate mass and time analyses of mass spectrometry data

被引：67

作者：

May, Damon ^{[1
]}

Fitzgibbon, Matt ^{[1
]}

Liu, Yan ^{[1
]}

Holzman, Ted ^{[1
]}

Eng, Jimmy ^{[1
]}

Kemp, C. J. ^{[1
]}

Whiteaker, Jeff ^{[1
]}

Paulovich, Amanda ^{[1
]}

McIntosh, Martin ^{[1
]}

机构：

[1] Fred Hutchinson Canc Res Ctr, Seattle, WA 98104 USA

来源：

JOURNAL OF PROTEOME RESEARCH | 2007年 / 6卷 / 07期

关键词：

CPAS; AMT; mslnspect; LC; MS; MS/MS; LC-MS; MODEL; IDENTIFICATIONS;

D O I：

10.1021/pr070146y

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe an integrated suite of algorithms and software for general accurate mass and time (AMT) tagging data analysis of mass spectrometry data. The AMT approach combines identifications from liquid chromatography (LC) tandem mass spectrometry (MS/MS) data with peptide accurate mass and retention time locations from high-resolution LC-MS data. Our workflow includes the traditional AMT approach, in which MS/MS identifications are located in external databases, as well as methods based on more recent hybrid instruments such as the LTQ-FT or Orbitrap, where MS/MS identifications are embedded with the MS data. We demonstrate our AMT workflow's utility for general data synthesis by combining data from two dissimilar biospecimens. Specifically, we demonstrate its use relevant to serum biomarker discovery by identifying which peptides sequenced by MS/MS analysis of tumor tissue may also be present in the plasma of tumor-bearing and control mice. The analysis workflow, referred to as msInspect/AMT, extends and combines existing open-source platforms for LC-MS/MS (CPAS) and LC-MS (msInspect) data analysis and is available in an unrestricted open-source distribution.

引用

页码：2685 / 2694

页数：10

共 20 条

[1] A suite of algorithms for the comprehensive analysis of complex protein mixtures using high-resolution LC-MS [J].