A novel electrochemical biosensor for ultrasensitive and specific detection of DNA based on molecular beacon mediated circular strand displacement and rolling circle amplification

被引：46

作者：

Cheng, Wei ^{[2
]}

Zhang, Wei ^{[1
]}

Yan, Yurong ^{[1
]}

Shen, Bo ^{[1
]}

Zhu, Dan ^{[1
]}

Lei, Pinhua ^{[1
]}

Ding, Shijia ^{[1
]}

机构：

[1] Chongqing Med Univ, Coll Lab Med, Key Lab Clin Lab Diagnost, Minist Educ, Chongqing 400016, Peoples R China

[2] Chongqing Med Univ, Affiliated Hosp 1, Ctr Clin Mol Med Detect, Chongqing 400016, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2014年 / 62卷

基金：

中国国家自然科学基金;

关键词：

Circular isothermal strand displacement amplification; Rolling circle amplification; Alkaline phosphatase; Electrochemical DNA sensor; CATALYZED SILVER DEPOSITION; REPLACEMENT POLYMERIZATION; SIGNAL AMPLIFICATION; NUCLEIC-ACIDS; SENSOR; OLIGONUCLEOTIDES; NANOPARTICLES; DNAZYME; OXIDE;

D O I：

10.1016/j.bios.2014.06.056

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 [生物物理学];

摘要：

A novel electrochemical biosensing strategy was developed for ultrasensitive and specific detection of target DNA using a cascade signal amplification based on molecular beacon (MB) mediated circular strand displacement (CSD), rolling circle amplification (RCA), biotin-strepavidin system, and enzymatic amplification. The target DNA hybridized with the loop portion of MB probe immobilized on the gold electrode and triggered the CSD, leading to multiple biotin-tagged DNA duplex. Furthermore, via biotin-streptavidin interaction, the RCA was implemented, producing long massive tandem-repeat DNA sequences for binding numerous biotinylated detection probes. This enabled an ultrasensitive electrochemical readout by further employing the streptavidin-alkaline phosphatase. The proposed biosensor showed very high sensitivity and selectivity with a dynamic response range from 1 fM to 100 pM. The proposed strategy could have the potential for applying in clinical molecular diagnostics and environmental monitoring. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：274 / 279

页数：6

共 34 条

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