Gracilibacillus boraciitolerans sp nov, a highly boron-tolerant and moderately halotolerant bacterium isolated from soil

被引:47
作者
Ahmed, Iftikhar
Yokota, Akira
Fujiwara, Toru
机构
[1] Univ Tokyo, Biotechnol Res Ctr, Bunkyo Ku, Tokyo 1138657, Japan
[2] Natl Agr Res Ctr, Islamabad 45500, Pakistan
[3] Univ Tokyo, Inst Mol & Cellular Biosci, Bunkyo Ku, Tokyo 1138657, Japan
[4] JST, SORST, Chiyoda Ku, Tokyo, Japan
关键词
D O I
10.1099/ijs.0.64284-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A motile, Gram-positive, boron-tolerant and moderately halotolerant rod-shaped bacterium was isolated from a soil naturally high in boron minerals found in the Hisarcik area of Turkey. The novel isolate, designated T-16X(T), produced spherical or ellipsoidal endospores in a non-bulging or slightly swollen sporangium in a terminal position and survived in a medium containing up to 450 mM boron. Whereas it tolerated 11 % (w/v) NaCl, it also grew without NaCl or boron. The temperature range for growth was 16-37 degrees C (optimum 25-28 degrees C and the pH range for growth was 6.0-10.0 (optimum pH 7.5-8.5). The DNA G+C content was 35.8 mol% and the major cellular fatty acids were iso-C-15:0 and anteiso-C-15:0 at 18.2 and 45.7 % of the total fatty acids, respectively. MK-7 (90%) was the predominant respiratory quinone system and mesodiaminopimelic acid was the predominant diamino acid of the cell-wall peptidoglycan. Phylogenetic analysis of the 16S rRNA gene sequence revealed that the novel strain is closely related to the type strains of Gracilibacillus orientalis (96.7 % similarity), G. halotolerans (95.5 %) and G, dipsosauri (95.4 %). However, the maximum DNA hybridization value for this strain with these closely related strains was less than 26.2 %. On the basis of 16S rRNA gene sequence data and chemotaxonomic and physiological features, the organism T-16X(T) (=DSM 17256(T) = IAM 15263(T) = ATCC BAA-1190(T)) is proposed to be a member of the genus Gracilibacillus as the type strain of the novel species Gracilibacillus boraciitolerans sp. nov.
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页码:796 / 802
页数:7
相关论文
共 35 条
[11]   Agrococcus jenensis gen nov, sp nov, a new genus of actinomycetes with diaminobutyric acid in the cell wall [J].
Groth, I ;
Schumann, P ;
Weiss, N ;
Martin, K ;
Rainey, FA .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1996, 46 (01) :234-239
[12]  
HALL T. A., 1999, Nucleic Acids Symp Ser, V41, P95, DOI [10.1021/bk-1999-0734.ch008, DOI 10.1021/BK-1999-0734.CH008]
[13]   THE TARTROLONS, NEW BORON-CONTAINING ANTIBIOTICS FROM A MYXOBACTERIUM, SORANGIUM CELLULOSUM [J].
IRSCHIK, H ;
SCHUMMER, D ;
GERTH, K ;
HOFLE, G ;
REICHENBACH, H .
JOURNAL OF ANTIBIOTICS, 1995, 48 (01) :26-30
[14]  
JONES BE, 1994, FEMS SYMP, P195
[15]   An extractive membrane biofilm reactor for degradation of 1,3-dichloropropene in industrial waste water [J].
Katsivela, E ;
Bonse, D ;
Krüger, A ;
Strömpl, C ;
Livingston, A ;
Wittich, RM .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1999, 52 (06) :853-862
[17]   Boromycin, an anti-HIV antibiotic [J].
Kohno, J ;
Kawahata, T ;
Otake, T ;
Morimoto, M ;
Mori, H ;
Ueba, N ;
Nishio, M ;
Kinumaki, A ;
Komatsubara, S ;
Kawashima, K .
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1996, 60 (06) :1036-1037
[18]  
Kudo T, 2001, IDENTIFICATION MANUA, P135
[19]   Phylogenetic characterization of a novel salt-tolerant Bacillus species: Description of Bacillus dipsosauri sp nov [J].
Lawson, PA ;
Deutch, CE ;
Collins, MD .
JOURNAL OF APPLIED BACTERIOLOGY, 1996, 81 (01) :109-112
[20]   AN INTEGRATED PROCEDURE FOR THE EXTRACTION OF BACTERIAL ISOPRENOID QUINONES AND POLAR LIPIDS [J].
MINNIKIN, DE ;
ODONNELL, AG ;
GOODFELLOW, M ;
ALDERSON, G ;
ATHALYE, M ;
SCHAAL, A ;
PARLETT, JH .
JOURNAL OF MICROBIOLOGICAL METHODS, 1984, 2 (05) :233-241