MiR-27b Impairs Adipocyte Differentiation of Human Adipose Tissue-Derived Mesenchymal Stem Cells by Targeting LPL (Publication with Expression of Concern. See vol. 57, pg. 158, 2023)

被引:34
作者
Hu, Xumin [1 ]
Tang, Jianhua [2 ]
Hu, Xuyun [3 ]
Bao, Peng [4 ]
Pan, Jinxin [1 ]
Chen, Zhipeng [1 ]
Xian, Jiaqi [1 ]
机构
[1] Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Dept Orthoped, 107 Yanjiangxi Rd, Guangzhou 510120, Guangdong, Peoples R China
[2] Peoples Hosp Gaozhou, Dept Orthoped, Gaozhou, Guangdong, Peoples R China
[3] Harvard Med Sch, Boston Childrens Hosp, Dept Newborn Med, Boston, MA USA
[4] Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Med Dept, Guangzhou, Guangdong, Peoples R China
关键词
Adipose tissue; Adipogenic differentiation; MiR-27b; Mesenchymal stem cells (MSCs); Metabolism; LPL; ADIPOGENIC DIFFERENTIATION; PPAR-GAMMA; SMALL MOLECULES; PROLIFERATION; SUPPRESSES; MICRORNAS; LINEAGE;
D O I
10.1159/000489988
中图分类号
Q2 [细胞生物学];
学科分类号
071013 [干细胞生物学];
摘要
Background/Aims: In this study, the molecular mechanisms of miR-27b and lipoprotein lipase (LPL) that regulate human adipose-derived mesenchymal stem cells (hASCs) adipogenic differentiation were detected. Methods: Microarray analysis was applied to screen for differentially expressed miRNAs and mRNA during hASCs adipocyte differentiation induction. MiR-27b and LPL were found to have abnormal expression. Then, a dual luciferase reporter assay was employed to validate the targeting relationship between miR-27b and LPL. We also utilized qRT-PCR, western blot, cellular immunofluorescence and an oil red O staining assay to analyze the regulation of miR-27b and LPL during adipogenic differentiation. Results: The microarray analysis demonstrated that, during adipogenic differentiation, miR-27b was downregulated, while LPL was up-regulated but tended to become stable 14 days after induction. A dual luciferase reporter assay confirmed the negative targeting regulatory relationship between miR-27b and LPL. After overexpressing and silencing miR-27b, LPL was found to be reversely regulated by miR-27b according to qRT-PCR and western blot. The fat-formationrelated biomarkers CCAAT-enhancer binding protein a (c/EBPa) and peroxisome proliferator activated receptors gamma (PPAR gamma) had decreasing levels after over-expressing miR-27b or knockdown of LPL followed by adipogenic differentiation. Meanwhile, the oil red O staining assay revealed that the accumulation of lipid droplets decreased. There was no change in the expression of c/EBPa, PPARy, or lipid droplet accumulation when overexpressing miR-27b and LPL. Conclusion: During the adipogenic differentiation of hASCs, miR-27b expression decreased, and LPL expression increased. The abnormal expression of miR-27b and LPL effectively regulated the adipogenic differentiation of hASCs. (C) 2018 The Author(s) Published by S. Karger AG, Basel
引用
收藏
页码:545 / 555
页数:11
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