Replication and Shedding of MERS-CoV in Upper Respiratory Tract of Inoculated Dromedary Camels

被引:194
作者
Adney, Danielle R. [1 ]
van Doremalen, Neeltje [2 ]
Brown, Vienna R. [1 ]
Bushmaker, Trenton [2 ]
Scott, Dana [2 ]
de Wit, Emmie [2 ]
Bowen, Richard A. [1 ]
Munster, Vincent J. [2 ]
机构
[1] Colorado State Univ, Ft Collins, CO 80523 USA
[2] NIH, Hamilton, MT USA
基金
美国国家卫生研究院;
关键词
SYNDROME CORONAVIRUS; SAUDI-ARABIA; INFECTION; ANTIBODIES; LIVESTOCK; JUNE;
D O I
10.3201/eid2012.141280
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
In 2012, a novel coronavirus associated with severe respiratory disease in humans emerged in the Middle East. Epidemiologic investigations identified dromedary camels as the likely source of zoonotic transmission of Middle East respiratory syndrome coronavirus (MERS-CoV). Here we provide experimental support for camels as a reservoir for MERS-CoV. We inoculated 3 adult camels with a human isolate of MERS-CoV and a transient, primarily upper respiratory tract infection developed in each of the 3 animals. Clinical signs of the MERS-CoV infection were benign, but each of the camels shed large quantities of virus from the upper respiratory tract. We detected infectious virus in nasal secretions through 7 days postinoculation, and viral RNA up to 35 days postinoculation. The pattern of shedding and propensity for the upper respiratory tract infection in dromedary camels may help explain the lack of systemic illness among naturally infected camels and the means of efficient camel-to-camel and camel-to-human transmission.
引用
收藏
页码:1999 / 2005
页数:7
相关论文
共 28 条
  • [11] A decade after SARS: strategies for controlling emerging coronaviruses
    Graham, Rachel L.
    Donaldson, Eric F.
    Baric, Ralph S.
    [J]. NATURE REVIEWS MICROBIOLOGY, 2013, 11 (12) : 836 - 848
  • [12] Middle East respiratory syndrome coronavirus in dromedary camels: an outbreak investigation
    Haagmans, Bart L.
    Al Dhahiry, Said H. S.
    Reusken, Chantal B. E. M.
    Raj, V. Stalin
    Galiano, Monica
    Myers, Richard
    Godeke, Gert-Jan
    Jonges, Marcel
    Farag, Elmoubasher
    Diab, Ayman
    Ghobashy, Hazem
    Alhajri, Farhoud
    Al-Thani, Mohamed
    Al-Marri, Salih A.
    Al Romaihi, Hamad E.
    Al Khal, Abdullatif
    Bermingham, Alison
    Osterhaus, Albert D. M. E.
    AlHajri, Mohd M.
    Koopmans, Marion P. G.
    [J]. LANCET INFECTIOUS DISEASES, 2014, 14 (02) : 140 - 145
  • [13] Middle East Respiratory Syndrome (MERS) coronavirus seroprevalence in domestic livestock in Saudi Arabia, 2010 to 2013
    Hemida, M. G.
    Perera, R. A.
    Wang, P.
    Alhammadi, M. A.
    Siu, L. Y.
    Li, M.
    Poon, L. L.
    Saif, L.
    Alnaeem, A.
    Peiris, M.
    [J]. EUROSURVEILLANCE, 2013, 18 (50): : 21 - 27
  • [14] MERS Coronavirus in Dromedary Camel Herd, Saudi Arabia
    Hemida, Maged G.
    Chu, Daniel K. W.
    Poon, Leo L. M.
    Perera, Ranawaka A. P. M.
    Alhammadi, Mohammad A.
    Hoi-yee Ng
    Siu, Lewis Y.
    Guan, Yi
    Alnaeem, Abdelmohsen
    Peiris, Malik
    [J]. EMERGING INFECTIOUS DISEASES, 2014, 20 (07) : 1231 - 1234
  • [15] Human Infection with MERS Coronavirus after Exposure to Infected Camels, Saudi Arabia, 2013
    Memish, Ziad A.
    Cotten, Matthew
    Meyer, Benjamin
    Watson, Simon J.
    Alsahafi, Abdullah J.
    Al Rabeeah, Abdullah A.
    Corman, Victor Max
    Sieberg, Andrea
    Makhdoom, Hatem Q.
    Assiri, Abdullah
    Al Masri, Malaki
    Aldabbagh, Souhaib
    Bosch, Berend-Jan
    Beer, Martin
    Mueller, Marcel A.
    Kellam, Paul
    Drosten, Christian
    [J]. EMERGING INFECTIOUS DISEASES, 2014, 20 (06) : 1012 - 1015
  • [16] Middle East Respiratory Syndrome Coronavirus in Bats, Saudi Arabia
    Memish, Ziad A.
    Mishra, Nischay
    Olival, Kevin J.
    Fagbo, Shamsudeen F.
    Kapoor, Vishal
    Epstein, Jonathan H.
    AlHakeem, Rafat
    Al Asmari, Mushabab
    Islam, Ariful
    Kapoor, Amit
    Briese, Thomas
    Daszak, Peter
    Al Rabeeah, Abdullah A.
    Lipkin, W. Ian
    [J]. EMERGING INFECTIOUS DISEASES, 2013, 19 (11) : 1819 - 1823
  • [17] Hospital-Associated Middle East Respiratory Syndrome Coronavirus Infections REPLY
    Memish, Ziad A.
    Al-Tawfiq, Jaffar A.
    Assiri, Abdullah
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 2013, 369 (18) : 1761 - 1762
  • [18] Antibodies against MERS Coronavirus in Dromedaries, United Arab Emirates, 2003 and 2013
    Meyer, Benjamin
    Mueller, Marcel A.
    Corman, Victor M.
    Reusken, Chantal B. E. M.
    Ritz, Daniel
    Godeke, Gert-Jan
    Lattwein, Erik
    Kallies, Stephan
    Siemens, Artem
    van Beek, Janko
    Drexler, Jan F.
    Muth, Doreen
    Bosch, Berend-Jan
    Wernery, Ulrich
    Koopmans, Marion P. G.
    Wernery, Renate
    Drosten, Christian
    [J]. EMERGING INFECTIOUS DISEASES, 2014, 20 (04) : 552 - 559
  • [19] The emergence of the Middle East Respiratory Syndrome coronavirus
    Milne-Price, Shauna
    Miazgowicz, Kerri L.
    Munster, Vincent J.
    [J]. PATHOGENS AND DISEASE, 2014, 71 (02): : 119 - 134
  • [20] Dynamics of passive immunity to West Nile virus in domestic chickens (Gallus gallus domesticus)
    Nemeth, Nicole M.
    Bowen, Richard A.
    [J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2007, 76 (02) : 310 - 317