Inhibition of influenza virus replication in cultured cells by RNA-cleaving DNA enzyme

被引：30

作者：

Toyoda, T

Imamura, Y

Takaku, H

Kashiwagi, T

Hara, K

Iwahashi, J

Ohtsu, Y

Tsumura, N

Kato, H

Hamada, N

机构：

[1] Kurume Univ, Sch Med, Dept Virol, Fukuoka 8300011, Japan

[2] Chiba Inst Technol, Dept Ind Chem, Narashino, Chiba 2750016, Japan

[3] Kurume Univ, Sch Med, Dept Pediat, Fukuoka 8300011, Japan

来源：

FEBS LETTERS | 2000年 / 481卷 / 02期

关键词：

influenza virus; RNA-cleaving DNA enzyme; antisense oligonucleotide; PB2;

D O I：

10.1016/S0014-5793(00)01974-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Influenza virus replication has been effectively inhibited by antisense phosphothioate oligonucleotides targeting the AUG initiation codon of PB2 mRNA, We designed RNA-cleaving DNA enzymes from 10-23 catalytic motif to target PB2-AUG initiation codon and measured their RNA-cleaving activity in vitro. Although the RNA-cleaving activity was not optimal under physiological conditions, DNA enzymes inhibited viral replication in cultured cells more effectively than antisense phosphothioate oligonucleotides. Our data indicated that DNA enzymes could be useful for the control of viral infection. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V, All rights reserved.

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页码：113 / 116

页数：4

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