Intronic miR-301 feedback regulates its host gene, ska2, in A549 cells by targeting MEOX2 to affect ERK/CREB pathways

被引：83

作者：

Cao, Guojun ^{[1
]}

Huang, Baochun ^{[1
]}

Liu, Zhaohui ^{[1
]}

Zhang, Jiyan ^{[1
]}

Xu, Hua ^{[1
]}

Xia, Wei ^{[1
]}

Li, Jie ^{[1
]}

Li, Shaohua ^{[1
]}

Chen, Liucun ^{[1
]}

Ding, Hongmei ^{[1
]}

Zhao, Qiang ^{[1
]}

Fan, Ming ^{[1
]}

Shen, Beifen ^{[1
]}

Shao, Ningsheng ^{[1
]}

机构：

[1] Beijing Inst Basic Med Sci, Beijing 100850, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 396卷 / 04期

关键词：

MicroRNA; miR-301; ska2; CREB; MICRORNA EXPRESSION; CANCER CELLS; GROWTH; SURVIVAL;

D O I：

10.1016/j.bbrc.2010.05.037

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

miR-301 is localized in the first intron of ska2, whose function has not been clarified. Here, a new circuit model in which intronic miR-301 regulates the transcription and function of its host gene through a feedback mechanism has been described. Our results showed that blocking of miR-301 in A549 cells leads to a decrease in the expression of the host gene, ska2. Further analysis showed that miR-301 targets MEOX2 to affect the ERK/CREB pathway. CREB directly regulates the expression of the host gene, ska2. In addition, the inhibition of miR-301 or ska2 resulted in an increase of the mitotic index and a decrease in colony formation in soft agar, which may be related to lung tumorigenesis. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：978 / 982

页数：5

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