Microfluidic sorting of mammalian cells by optical force switching

被引：581

作者：

Wang, MM ^{[1
]}

Tu, E ^{[1
]}

Raymond, DE ^{[1
]}

Yang, JM ^{[1
]}

Zhang, HC ^{[1
]}

Hagen, N ^{[1
]}

Dees, B ^{[1
]}

Mercer, EM ^{[1
]}

Forster, AH ^{[1
]}

Kariv, I ^{[1
]}

Marchand, PJ ^{[1
]}

Butler, WF ^{[1
]}

机构：

[1] Genoptix Inc, San Diego, CA 92121 USA

来源：

NATURE BIOTECHNOLOGY | 2005年 / 23卷 / 01期

关键词：

D O I：

10.1038/nbt1050

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Microfluidic-based devices have allowed miniaturization and increased parallelism of many common functions in biological assays; however, development of a practical technology for microfluidic-based fluorescence-activated cell sorting has proved challenging. Although a variety of different physical on-chip switch mechanisms have been proposed(1-6), none has satisfied simultaneously the requirements of high throughput, purity, and recovery of live, unstressed mammalian cells. Here we show that optical forces can be used for the rapid (2-4 ms), active control of cell routing on a microfluidic chip. Optical switch controls reduce the complexity of the chip and simplify connectivity. Using all-optical switching, we have implemented a fluorescence-activated microfluidic cell sorter and evaluated its performance on live, stably transfected HeLa cells expressing a fused histone-green fluorescent protein. Recovered populations were verified to be both viable and unstressed by evaluation of the transcriptional expression of two genes, HSPA6 and FOS, known indicators of cellular stress.

引用

页码：83 / 87

页数：5

共 36 条

[1] OPTICAL TRAPPING AND MANIPULATION OF VIRUSES AND BACTERIA [J].